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Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila

文献类型：期刊论文

作者Maa, Yang1,2,5; Yen, Guanxiong1,2,5; Han, Xiaojie3,5; Zhang, Jing5; Xiong, Jie5; Miao, Wei1,2,4,5
刊名JOURNAL OF CELL SCIENCE
出版日期2020-03-01
卷号133期号:6页码:12
关键词Cell cycle Cyclin Cyclin-dependent kinase Sexual reproduction Tetrahymena thermophila
ISSN号0021-9533
DOI10.1242/jcs.235721
通讯作者Miao, Wei(miaowei@ihb.ac.cn)
英文摘要To investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila. We identified two genes, CDK19 and CYC9, which are highly co-expressed with the mating-associated factors MTA, MTB and HAP2. Both CDK19 and CYC9 were found to be essential for mating in T. thermophila. Subcellular localization experiments suggested that these proteins are located at the oral area, including the conjugation junction area, and that CDK19 or CYC9 knockout prevents mating. We found that CDK19 and CYC9 form a complex, and also identified several additional subunits, which may have regulatory or constitutive functions. RNA sequencing analyses and cytological experiments showed that mating is abnormal in both Delta CDK19 and Delta CYC9, mainly at the entry to the co-stimulation stage. These results indicate that the CDK19-CYC9 complex initiates the sexual cell cycle in T. thermophila.
WOS关键词DEVELOPMENTAL INTERACTION ; DEPENDENT KINASE ; CO-STIMULATION ; PAIR FORMATION ; GENE ONTOLOGY ; PROTEIN ; TRANSFORMATION ; CONJUGATION ; MEIOSIS ; CLASSIFICATION
资助项目National Natural Science Foundation of China[91631303] ; National Natural Science Foundation of China[31525021]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000534387000010
出版者COMPANY BIOLOGISTS LTD
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/36534]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Miao, Wei
作者单位1.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
4.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodiversily & Conservat, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Maa, Yang,Yen, Guanxiong,Han, Xiaojie,et al. Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila[J]. JOURNAL OF CELL SCIENCE,2020,133(6):12.
APA Maa, Yang,Yen, Guanxiong,Han, Xiaojie,Zhang, Jing,Xiong, Jie,&Miao, Wei.(2020).Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila.JOURNAL OF CELL SCIENCE,133(6),12.
MLA Maa, Yang,et al."Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila".JOURNAL OF CELL SCIENCE 133.6(2020):12.

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来源：水生生物研究所

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