Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)
文献类型：期刊论文
| 作者 | Li, Gaojie1,2; Hu, Shiqi1,2; Yang, Jingjing1,2; Zhao, Xuyao1,2; Kimura, Seisuke3,4; Schultz, Elizabeth A.5; Hou, Hongwei1,2 |
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2020-06-01 |
| 卷号 | 39期号:6页码:737-750 |
| 关键词 | Agrobacterium tumefaciens Cytokinin Heterophylly Hygrophila difformis Transformation Leaf form |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-020-02527-x |
| 通讯作者 | Hou, Hongwei(houhw@ihb.ac.cn) |
| 英文摘要 | Key message This is the first report of a highly efficient Agrobacterium tumefaciens-mediated transformation protocol for Acanthaceae and its utilization in revealing important roles of cytokinin in regulating heterophylly in Hygrophila difformis. Plants show amazing morphological differences in leaf form in response to changes in the surrounding environment, which is a phenomenon called heterophylly. Previous studies have shown that the aquatic plant Hygrophila difformis (Acanthaceae) is an ideal model for heterophylly study. However, low efficiency and poor reproducibility of genetic transformation restricted H. difformis as a model plant. In this study, we reported successful induction of callus, shoots and the establishment of an efficient stable transformation protocol as mediated by Agrobacterium tumefaciens LBA4404. We found that the highest callus induction efficiency was achieved with 1 mg/L 1-Naphthaleneacetic acid (NAA) and 2 mg/L 6-benzyladenine (6-BA), that efficient shoot induction required 0.1 mg/L NAA and 0.1 mg/L 6-BA and that high transformation efficiency required 100 mu M acetosyringone. Due to the importance of phytohormones in the regulation of heterophylly and the inadequate knowledge about the function of cytokinin (CK) in this process, we analyzed the function of CK in the regulation of heterophylly by exogenous CK application and endogenous CK detection. By using our newly developed transformation system to detect CK signals, contents and distribution in H. difformis, we revealed an important role of CK in environmental mediated heterophylly. |
| WOS关键词 | CARBON-DIOXIDE ; ABSCISIC-ACID ; LEAF ; INDUCTION ; MORPHOGENESIS ; GIBBERELLIN ; MECHANISMS ; ETHYLENE ; EXPLANTS ; GROWTH |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000533794600005 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/36619]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 通讯作者 | Hou, Hongwei |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Kyoto Sangyo Univ, Fac Life Sci, Kita Ku, Kyoto, Kyoto 6038555, Japan 4.Kyoto Sangyo Univ, Ctr Ecol Evolutionary Dev Biol, Kita Ku, Kyoto, Kyoto 6038555, Japan 5.Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada |
| 推荐引用方式 GB/T 7714 | Li, Gaojie,Hu, Shiqi,Yang, Jingjing,et al. Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)[J]. PLANT CELL REPORTS,2020,39(6):737-750. |
| APA | Li, Gaojie.,Hu, Shiqi.,Yang, Jingjing.,Zhao, Xuyao.,Kimura, Seisuke.,...&Hou, Hongwei.(2020).Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae).PLANT CELL REPORTS,39(6),737-750. |
| MLA | Li, Gaojie,et al."Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae)".PLANT CELL REPORTS 39.6(2020):737-750. |
入库方式： OAI收割
来源：水生生物研究所
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