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  5. 期刊论文
The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10

文献类型：期刊论文

作者Yang, Xue1,2,3; Li, Siting1,2,3; Wu, Ying1,2,3; Ge, Feng1,2,3; Chen, Ying4; Xiong, Qian1,2,3
刊名BMC CANCER
出版日期2020-04-15
卷号20期号:1页码:12
关键词Circular RNAs CDR1as Quantitative proteomics TMED2 TMED10 miR-7
DOI10.1186/s12885-020-06794-5
通讯作者Xiong, Qian(xiongqian@ihb.ac.cn)
英文摘要BackgroundCircular RNAs (CircRNAs) are biologically active RNAs. CDR1as is one such circRNA previously reported to be a microRNA-7 (miR-7) sponge, thereby regulating associated gene expression. The specific underlying molecular mechanisms of CDR1as biology, however, remain largely unknown.MethodsWe performed CDR1as knockdown in order to explore its function in cell proliferation, migration, the cell cycle, and tumorigenesis. We further employed quantitative proteomic analyses and associated bioinformatics strategies to globally assess CDR1as-regulated proteins (CRPs). Western blotting and immunofluorescence staining were used to validate the proteomic results. We additionally investigated a specific link between TMED2, TMED10, and miR-7 via a dual-luciferase reporter system, and generated CDR1as knockout cell lines via CRISPR/Cas9 editing.ResultsWe identified 353 proteins dysregulated upon CDR1as knockdown in 293T cells. These CRPs were found to interact with one another and to play key roles in certain cellular pathways. Two such proteins, TMED2 and TMED10, were found to specifically contribute to the influence of CDR1as on cell proliferation. CDR1as may regulate these two TMED proteins through miR-7 sponging. We were able to further confirm these results using both CRISPRi cell lines and nude mouse models.ConclusionThis study suggested that CDR1as may regulate cell proliferation via serving as a miR-7 sponge, thereby regulating TMED2 and TMED10 expression. These results are an invaluable template for future streamlined studies of circRNAs.
WOS关键词NONCODING RNA ; CANCER ; EXPRESSION ; PROMOTE ; MIR-7 ; TMP21 ; GENE ; TRAFFICKING ; PROGRESSION ; MICRORNA-7
资助项目National Natural Science Foundation of China[31870756] ; National Natural Science Foundation of China[91540102] ; National Natural Science Foundation of China[31601036] ; National Key Research and Development Program[2016YFA0501304] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB14030202]
WOS研究方向Oncology
语种英语
WOS记录号WOS:000531187700011
出版者BMC
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program ; National Key Research and Development Program ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program ; National Key Research and Development Program ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program ; National Key Research and Development Program ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program ; National Key Research and Development Program ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences
源URL[http://ir.ihb.ac.cn/handle/342005/35796]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Xiong, Qian
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Yangtze Univ, Coll Life Sci, Jingzhou 434025, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Xue,Li, Siting,Wu, Ying,et al. The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10[J]. BMC CANCER,2020,20(1):12.
APA Yang, Xue,Li, Siting,Wu, Ying,Ge, Feng,Chen, Ying,&Xiong, Qian.(2020).The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.BMC CANCER,20(1),12.
MLA Yang, Xue,et al."The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10".BMC CANCER 20.1(2020):12.

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来源：水生生物研究所

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