中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 藻类生物学及应用研究中心
  5. 期刊论文
Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins

文献类型：期刊论文

作者Wang, Yali1,4; Liu, Xiaoxiang4; Gao, Hong1,4; Zhang, Hong-Mei2; Guo, An-Yuan2; Xu, Jian3; Xu, Xudong1,4
刊名MOLECULAR BIOLOGY AND EVOLUTION
出版日期2020-03-01
卷号37期号:3页码:849-863
ISSN号0737-4038
关键词Antarctica cold adaptation intraspecies divergence omics enzyme activity Chlorella vulgaris
DOI10.1093/molbev/msz273
通讯作者Xu, Xudong(xux@ihb.ac.cn)
英文摘要It is known that adaptive evolution in permanently cold environments drives cold adaptation in enzymes. However, how the relatively high enzyme activities were achieved in cold environments prior to cold adaptation of enzymes is unclear. Here we report that an Antarctic strain of Chlorella vulgaris, called NJ-7, acquired the capability to grow at near 0 degrees C temperatures and greatly enhanced freezing tolerance after systematic increases in abundance of enzymes/proteins and positive selection of certain genes. Having diverged from the temperate strain UTEX259 of the same species 2.5 (1.1-4.1) to 2.6 (1.0-4.5) Ma, NJ-7 retained the basic mesophilic characteristics and genome structures. Nitrate reductases in the two strains are highly similar in amino acid sequence and optimal temperature, but the NJ-7 one showed significantly higher abundance and activity. Quantitative proteomic analyses indicated that several cryoprotective proteins (LEA), many enzymes involved in carbon metabolism and a large number of other enzymes/proteins, were more abundant in NJ-7 than in UTEX259. Like nitrate reductase, most of these enzymes were not upregulated in response to cold stress. Thus, compensation of low specific activities by increased enzyme abundance appears to be an important strategy for early stage cold adaptation to Antarctica, but such enzymes are mostly not involved in cold acclimation upon transfer from favorable temperatures to near 0 degrees C temperatures.
WOS关键词CHLORELLA-VULGARIS C-27 ; FREEZING TOLERANCE ; TEMPERATE STRAINS ; IDENTIFICATION ; EMBRYOGENESIS ; ICE ; GENES ; COLD ; EVOLUTION ; MICROORGANISMS
资助项目Knowledge Innovation Project of Hubei Province[2017CFA021] ; STS Project of Chinese Academy of Sciences[KFJ-SW-STS-163] ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ06]
WOS研究方向Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
语种英语
出版者OXFORD UNIV PRESS
WOS记录号WOS:000522244100018
资助机构Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS
源URL[http://ir.ihb.ac.cn/handle/342005/35915]  
专题水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者Xu, Xudong
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
2.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yali,Liu, Xiaoxiang,Gao, Hong,et al. Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins[J]. MOLECULAR BIOLOGY AND EVOLUTION,2020,37(3):849-863.
APA Wang, Yali.,Liu, Xiaoxiang.,Gao, Hong.,Zhang, Hong-Mei.,Guo, An-Yuan.,...&Xu, Xudong.(2020).Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins.MOLECULAR BIOLOGY AND EVOLUTION,37(3),849-863.
MLA Wang, Yali,et al."Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins".MOLECULAR BIOLOGY AND EVOLUTION 37.3(2020):849-863.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。