Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis
文献类型：期刊论文
| 作者 | Jiang, Yongguang2; Chen, Youxin1; Yang, Shimin2; Li, Renhui1 |
| 刊名 | HARMFUL ALGAE
 |
| 出版日期 | 2020-03-01 |
| 卷号 | 93页码:11 |
| 关键词 | Cyanotoxin Saxitoxin Cylindrospermopsin Biosynthesis Gene cluster Phylogeny |
| ISSN号 | 1568-9883 |
| DOI | 10.1016/j.hal.2020.101792 |
| 通讯作者 | Li, Renhui(reli@ihb.ac.cn) |
| 英文摘要 | Cylindrospermopsis and Raphidiopsis (C/R group) are closely related species responsible for cyanobacterial blooms worldwide. Paralytic shellfish toxins (PSTs) and cylindrospermopsins (CYNs) have been identified in different C/R group strains. However, the evolutionary relationship between PST- and CYN-producing strains has not been systematically evaluated. In this study, C/R group strains and their toxin biosynthesis genes were evaluated by phylogenetic analysis and sequence comparison. None of the tested strains are able to produce PSTs and CYNs simultaneously. The C/R group strains were clustered into five clades, including two non-toxic, two CYN-producing and one PST-producing clades. A high degree of similarity was observed for rpoC1 (> 96%) and ITS-L (> 97%) sequences within each clade with the exception of the ITS-L (87% to 100%) region in CYN-producing R. curvata, which has been shown to contain variable sequence insertions. Genomic analysis revealed that sxtY and sxtZ could be found in both toxic and non-toxic strains. The transposase gene IS4 was only observed in strains from the PST-producing clade. The sxt and cyr gene clusters share five gene families with similar functions. The amino acid sequences of the adenylyl-sulfate kinase genes, sxtO and cyrN, are more similar (45% to 81%) than other pairs of genes (8.0% to 40%). SxtO and CyrN proteins from C/R group strains forms an independent clade on the phylogenetic tree with a high degree of sequence similarity (78% to 100%). In conclusion, PST- and CYN-producing C/R group species can be classified into different clades based on their phylogenetic profile. The sxtO and cyrN genes have probably diverged from a single ancestral adenylyl-sulfate kinase gene, and may be specifically used for toxin biosynthesis in C/R group species. |
| WOS关键词 | CYANOBACTERIAL GENERA CYLINDROSPERMOPSIS ; RACIBORSKII ; BIOSYNTHESIS ; CLUSTER ; STRAIN ; PHYLOGEOGRAPHY ; NOSTOCALES ; DEOXYCYLINDROSPERMOPSIN ; DIVERSITY |
| 资助项目 | National Key Research and Development Program of China[2018YFA0901300] ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences[Y85Z061601] ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000527331100011 |
| 出版者 | ELSEVIER |
| 资助机构 | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Featured Institute Service Projects from the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) ; Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/36410]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 通讯作者 | Li, Renhui |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.China Univ Geosci, Sch Environm Studies, Dept Biol Sci & Technol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yongguang,Chen, Youxin,Yang, Shimin,et al. Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis[J]. HARMFUL ALGAE,2020,93:11. |
| APA | Jiang, Yongguang,Chen, Youxin,Yang, Shimin,&Li, Renhui.(2020).Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis.HARMFUL ALGAE,93,11. |
| MLA | Jiang, Yongguang,et al."Phylogenetic relationships and genetic divergence of paralytic shellfish toxin- and cylindrospermopsin- producing Cylindrospermopsis and raphidiopsis".HARMFUL ALGAE 93(2020):11. |
入库方式： OAI收割
来源：水生生物研究所
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