Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)
文献类型：期刊论文
| 作者 | Xiong, Fan1,2; Qin, Lu1,2; Hao, Yao-tong3; Zhao, Di1,2; Li, Wen-xiang1,2; Zou, Hong1,2; Li, Ming1,2; Wu, Shan-gong1,2; Wang, Gui-tang1,2 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2020-05-15 |
| 卷号 | 521页码:9 |
| 关键词 | Gut microbiota Citrobacter freundii Immune response Goblet cells Grass carp |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2020.735015 |
| 通讯作者 | Xiong, Fan() ; Wu, Shan-gong(wusgz@ihb.ac.cn) |
| 英文摘要 | The effect of Citrobacter freundii, an opportunistic pathogen of fish, on the gut microbiota and immune responses in fish remains unknown. We fed grass carp (Ctenopharyngodon idellus) with two different concentrations of this bacterium (experimental Low and High groups, plus Control group). No signs of acute inflammatory response were found in either of the experimental groups using the intestinal HE staining method, but the number of intestinal goblet cells was much higher in the High group. Furthermore, we found that feeding C. freundii significantly decreased the alpha diversity and altered beta diversity of gut microbiota in both groups, and significantly promoted the relative abundance of Enterobacteriales, Pasteurellales, Neisseriales and Citrobacter in the High group. Spleen transcriptome showed that the expression pattern of immune system-related genes varied significantly between the Control group and High group. Several immunity-related pathways and two infectious diseases-related pathways were significantly enriched in the High group. Many complement system genes, including c3, c4, c5, c7, c8a, c8b and c1s, were also up-regulated in the High group. Overall, our results indicate that C. freundii did not trigger acute intestinal inflammation, but it changed the diversity and population structure of gut microbiota, and triggered the host's innate mucosal immune system. The enrichment of two infectious diseases-related pathways also suggested a higher risk of infection in the High group. These results contribute to our understanding of the effects of opportunistic fish pathogens on gut microbiota and host's immune responses. |
| WOS关键词 | COMPLEMENT ; INFECTION ; STRINGTIE ; SYSTEM ; HEALTH ; HISAT |
| 资助项目 | National Natural Science Foundation of China[31872612] ; National Key R&D Program of China[2018YFD0900400] ; earmarked fund for China Agriculture Research System[CARS-45-15] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000521282400042 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/35983]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 通讯作者 | Xiong, Fan; Wu, Shan-gong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr,State Key Lab Freshwater Ecol & Biotec, Wuhan, Peoples R China 3.Hebei Agr Univ, Ocean Coll, Qinhuangdao, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Fan,Qin, Lu,Hao, Yao-tong,et al. Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)[J]. AQUACULTURE,2020,521:9. |
| APA | Xiong, Fan.,Qin, Lu.,Hao, Yao-tong.,Zhao, Di.,Li, Wen-xiang.,...&Wang, Gui-tang.(2020).Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus).AQUACULTURE,521,9. |
| MLA | Xiong, Fan,et al."Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)".AQUACULTURE 521(2020):9. |
入库方式： OAI收割
来源：水生生物研究所
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