Chromosome-level analysis of the Crassostrea hongkongensis genome reveals extensive duplication of immune-related genes in bivalves
文献类型:期刊论文
作者 | Peng, Jinxia3; Li, Qiongzhen3; Xu, Lian2,4; Wei, Pinyuan3; He, Pingping3; Zhang, Xingzhi3; Zhang, Li3; Guan, Junliang3; Zhang, Xiaojuan1,5; Lin, Yong3 |
刊名 | MOLECULAR ECOLOGY RESOURCES |
出版日期 | 2020-04-15 |
期号 | 1页码:15 |
ISSN号 | 1755-098X |
关键词 | adaptation bivalve Crassostrea hongkongensis genome gonad development transcriptome |
DOI | 10.1111/1755-0998.13157 |
英文摘要 | Crassostrea hongkongensis is a popular and important native oyster species that is cultured mainly along the coast of the South China Sea. However, the absence of a reference genome has restricted genetic studies and the development of molecular breeding schemes for this species. Here, we combined PacBio and 10 x Genomics technologies to create a C. hongkongensis genome assembly, which has a size of 610 Mb, and is close to that estimated by flow cytometry (similar to 650 Mb). Contig and scaffold N50 are 2.57 and 4.99 Mb, respectively, and BUSCO analysis indicates that 95.8% of metazoan conserved genes are completely represented. Using a high-density linkage map of its closest related species, C. gigas, a total of 521 Mb (85.4%) was anchored to 10 haploid chromosomes. Comparative genomic analyses with other molluscs reveal that several immune- or stress response-related genes extensively expanded in bivalves by tandem duplication, including C1q, Toll-like receptors and Hsp70, which are associated with their adaptation to filter-feeding and sessile lifestyles in shallow sea and/or deep-sea ecosystems. Through transcriptome sequencing, potential genes and pathways related to sex determination and gonad development were identified. The genome and transcriptome of C. hongkongensis provide valuable resources for future molecular studies, genetic improvement and genome-assisted breeding of oysters. |
WOS关键词 | SEX DETERMINATION ; DIVERGENCE TIMES ; IDENTIFICATION ; SEQUENCE ; ALIGNMENT ; OYSTERS ; EVOLUTION ; PROGRAM ; INVOLVEMENT ; PREDICTION |
WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000525824400001 |
源URL | [http://ir.ihb.ac.cn/handle/342005/36091] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Lin, Yong; Gui, Jianfang; Chen, Xiaohan |
作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan, Peoples R China 2.Nantong Univ, Coinnovat Ctr Neuroregenerat, Key Lab Neuroregenerat Jiangsu, Nantong, Peoples R China 3.Guangxi Acad Fisheries Sci, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning, Peoples R China 4.Nantong Univ, Coinnovat Ctr Neuroregenerat, Minist Educ, Nantong, Peoples R China 5.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Peng, Jinxia,Li, Qiongzhen,Xu, Lian,et al. Chromosome-level analysis of the Crassostrea hongkongensis genome reveals extensive duplication of immune-related genes in bivalves[J]. MOLECULAR ECOLOGY RESOURCES,2020(1):15. |
APA | Peng, Jinxia.,Li, Qiongzhen.,Xu, Lian.,Wei, Pinyuan.,He, Pingping.,...&Chen, Xiaohan.(2020).Chromosome-level analysis of the Crassostrea hongkongensis genome reveals extensive duplication of immune-related genes in bivalves.MOLECULAR ECOLOGY RESOURCES(1),15. |
MLA | Peng, Jinxia,et al."Chromosome-level analysis of the Crassostrea hongkongensis genome reveals extensive duplication of immune-related genes in bivalves".MOLECULAR ECOLOGY RESOURCES .1(2020):15. |
入库方式: OAI收割
来源:水生生物研究所
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