Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina
文献类型：期刊论文
| 作者 | Zhao, Weishan2,3,4; Hu, Guangran2,3,4; Ponce-Gordo, Francisco1; Zou, Hong2,3; Li, Wenxiang2,3; Wu, Shangong2,3; Li, Ming2,3; Wang, Guitang2,3 |
| 刊名 | PARASITOLOGY INTERNATIONAL
 |
| 出版日期 | 2020-06-01 |
| 卷号 | 76页码:7 |
| 关键词 | Opalina obtrigonoidea Duttaphrynus melanostictus Morphology SSU rDNA ITS region |
| ISSN号 | 1383-5769 |
| DOI | 10.1016/j.parint.2020.102103 |
| 通讯作者 | Li, Ming(liming@ihb.ac.cn) |
| 英文摘要 | The redescription of Opalina obtrigonoidea Metcalf, 1923, collected from the rectum of the toads Duttaphrynus melanostictus, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that O. obtrigonoidea varies greatly in body dimensions. Its morphological characteristics allow its differentiation from Opalina undulata. Surprisingly, we sequenced its SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA (5' end) and found the SSU rDNA of O. obtrigonoidea is nearly identical to that of O. undulata. However, there are differences in both the ITS1 and ITS2 regions that allow their distinction and confirm the morphological differences. Our results indicate that O. obtrigonoidea and O. undulata are closely related species in which morphological and genetic markers have evolved at different speeds. Due to this, the SSU rDNA gene may not be a valid marker for inter-species identification in Opalina, but the ITS is a valid marker for differentiating species in this genus. |
| WOS关键词 | CLUSTAL-W ; ITS2 ; PROTOZOA |
| 资助项目 | National Natural Science Foundation of China[31772429] ; Youth Innovation Promotion Association CAS[Y82Z01] ; second Tibetan Plateau Scientific Expedition and Research Program (STEP)[2019QZKK0304] ; earmarked fund for China Agriculture Research System[CARS-45-15] |
| WOS研究方向 | Parasitology |
| 语种 | 英语 |
| WOS记录号 | WOS:000528164100045 |
| 出版者 | ELSEVIER IRELAND LTD |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; second Tibetan Plateau Scientific Expedition and Research Program (STEP) ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/36416]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 通讯作者 | Li, Ming |
| 作者单位 | 1.Univ Complutense Madrid, Fac Farm, Dept Microbiol & Parasitol, Plaza Ramony Cajal S-N, E-28040 Madrid, Spain 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Minist Agr, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Weishan,Hu, Guangran,Ponce-Gordo, Francisco,et al. Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina[J]. PARASITOLOGY INTERNATIONAL,2020,76:7. |
| APA | Zhao, Weishan.,Hu, Guangran.,Ponce-Gordo, Francisco.,Zou, Hong.,Li, Wenxiang.,...&Wang, Guitang.(2020).Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina.PARASITOLOGY INTERNATIONAL,76,7. |
| MLA | Zhao, Weishan,et al."Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina".PARASITOLOGY INTERNATIONAL 76(2020):7. |
入库方式： OAI收割
来源：水生生物研究所
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