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Andrias davidianus ranavirus 1R encoding a delayed-early protein promotes cell proliferation by driving cell cycle progression into S phase

文献类型：期刊论文

作者Yu, N-T1,2; Ke, F.1; Zhang, Q-Y1,2
刊名ACTA VIROLOGICA
出版日期2020
卷号64期号:1页码:10-19
关键词ranavirus Andrias davidianus ranavirus core gene cell cycle cell proliferations
ISSN号0001-723X
DOI10.4149/av_2020_102
通讯作者Zhang, Q-Y(zhangqy@ihb.ac.cn)
英文摘要Andrias davidianus ranavirus 1R (ADRV-1R), a core gene of the family Iridoviridae, is predicted to encode a viral transcription factor (vTF) since the protein contains a virus late transcription factor-3 like (VLTF3 like) domain. However, its characteristics and function are still unclear. In this study, the transcription and expression of ADRV-1R were investigated in Chinese giant salamander thymus cells (GSTCs). ADRV-1R transcription starts 6 hours post-infection (hpi), while the protein expression starts 8 hpi. Drug inhibition assay showed that the transcripts are inhibited by cycloheximide (CHX), a de novo protein synthesis inhibitor, indicating that ADRV-1R is a viral delayed-early (DE) gene. Subcellular localization showed that ADRV-1R is distributed in the cell nucleus and cytoplasm. The effect of ADRV-1R overexpression on cell proliferation and virus titer was analyzed. ADRV-1R overexpression significantly promoted the cell proliferation starting at day 2. Flow cytometry analysis further indicated that the protein promotes the GSTC cell cycle progression from GI phase into S phase (Gl/S transition). Moreover, ADRV-1R overexperession significantly increased ADRV titer in GSTCs. The virus titer was 6.3-6.9-fold higher at 36 hpi and further after than the control GSTC lines. These data showed that ADRV-1R is a delayed-early protein promoting cell proliferation and virus titers.
WOS关键词CHINESE GIANT SALAMANDER ; TRANSCRIPTION FACTOR ; RANA-GRYLIO ; IRIDOVIRUS ; EXPRESSION
资助项目National Natural Science Foundation of China[31430091]
WOS研究方向Virology
语种英语
WOS记录号WOS:000534481100002
出版者AEPRESS SRO
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/36548]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Zhang, Q-Y
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yu, N-T,Ke, F.,Zhang, Q-Y. Andrias davidianus ranavirus 1R encoding a delayed-early protein promotes cell proliferation by driving cell cycle progression into S phase[J]. ACTA VIROLOGICA,2020,64(1):10-19.
APA Yu, N-T,Ke, F.,&Zhang, Q-Y.(2020).Andrias davidianus ranavirus 1R encoding a delayed-early protein promotes cell proliferation by driving cell cycle progression into S phase.ACTA VIROLOGICA,64(1),10-19.
MLA Yu, N-T,et al."Andrias davidianus ranavirus 1R encoding a delayed-early protein promotes cell proliferation by driving cell cycle progression into S phase".ACTA VIROLOGICA 64.1(2020):10-19.

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来源：水生生物研究所

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