Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation
文献类型：期刊论文
| 作者 | Mohammed, Ibrahim1; Ijaz, Sahar1; Mokhtari, Tahmineh3,4; Gholaminejhad, Morteza1; Mahdavipour, Marzieh1; Jameie, Behnamedin2; Akbari, Mohammad1; Hassanzadeh, Gholamreza1 |
| 刊名 | METABOLIC BRAIN DISEASE
 |
| 出版日期 | 2020-03-17 |
| 页码 | 10 |
| ISSN号 | 0885-7490 |
| 关键词 | Sub-ventricular zone Extracellular vesicle Inflammasome Spinal cord injury Rat |
| DOI | 10.1007/s11011-020-00563-w |
| 通讯作者 | Hassanzadeh, Gholamreza(hassanzadeh@tums.ac.ir) |
| 英文摘要 | Spinal cord injury (SCI) is the destruction of spinal cord motor and sensory resulted from an attack on the spinal cord, which can cause significant physiological damage. The inflammasome is a multiprotein oligomer resulting in inflammation; the NLRP3 inflammasome composed of NLRP3, apoptosis-associated speck-like protein (ASC), procaspase-1, and cleavage of procaspase-1 into caspase-1 initiates the inflammatory response. Subventricular Zone (SVZ) is the origin of neural stem/progenitor cells (NS/PCs) in the adult brain. Extracellular vesicles (EVs) are tiny lipid membrane bilayer vesicles secreted by different types of cells playing an important role in cell-cell communications. The aim of this study was to investigate the effect of intrathecal transplantation of EVs on the NLRP3 inflammasome formation in SCI rats. Male wistar rats were divided into three groups as following: laminectotomy group, SCI group, and EVs group. EVs was isolated from SVZ, and characterized by western blot and DLS, and then injected into the SCI rats. Real-time PCR and western blot were carried out for gene expression and protein level of NLRP3, ASC, and Caspase-1. H&E and cresyl violet staining were performed for histological analyses, as well as BBB test for motor function. The results indicated high level in mRNA and protein level in SCI group in comparison with laminectomy (p < 0.001), and injection of EVs showed a significant reduction in the mRNA and protein levels in EVs group compared to SCI (p < 0.001). H&E and cresyl violet staining showed recovery in neural cells of spinal cord tissue in EVs group in comparison with SCI group. BBB test showed the promotion of motor function in EVs group compared to SCI in 14 days (p < 0.05). We concluded that the injection of EVs could recover the motor function in rats with SCI and rescue the neural cells of spinal cord tissue by suppressing the formation of the NLRP3 inflammasome complex. |
| WOS关键词 | STROMAL CELLS ; STEM-CELLS ; NEUROVASCULAR PLASTICITY ; CURRENT STATE ; BRAIN ; TRANSPLANTATION ; DELIVERY ; MICROPARTICLES ; ACTIVATION ; PLATFORM |
| 资助项目 | Tehran University of Medical Sciences[97-01-103-37391] |
| WOS研究方向 | Endocrinology & Metabolism ; Neurosciences & Neurology |
| 语种 | 英语 |
| 出版者 | SPRINGER/PLENUM PUBLISHERS |
| WOS记录号 | WOS:000520669000001 |
| 资助机构 | Tehran University of Medical Sciences |
| 源URL | [http://ir.psych.ac.cn/handle/311026/31491]  |
| 专题 | 心理研究所_中国科学院心理健康重点实验室 |
| 通讯作者 | Hassanzadeh, Gholamreza |
| 作者单位 | 1.Univ Tehran Med Sci, Sch Med, Dept Anat, Tehran, Iran 2.Iran Univ Med Sci, Neurosci Res Ctr, Tehran, Iran 3.Univ Chinese Acad Sci, Dept Psychol, Beijing, Peoples R China 4.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mohammed, Ibrahim,Ijaz, Sahar,Mokhtari, Tahmineh,et al. Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation[J]. METABOLIC BRAIN DISEASE,2020:10. |
| APA | Mohammed, Ibrahim.,Ijaz, Sahar.,Mokhtari, Tahmineh.,Gholaminejhad, Morteza.,Mahdavipour, Marzieh.,...&Hassanzadeh, Gholamreza.(2020).Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation.METABOLIC BRAIN DISEASE,10. |
| MLA | Mohammed, Ibrahim,et al."Subventricular zone-derived extracellular vesicles promote functional recovery in rat model of spinal cord injury by inhibition of NLRP3 inflammasome complex formation".METABOLIC BRAIN DISEASE (2020):10. |
入库方式： OAI收割
来源：心理研究所
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