bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis
文献类型：期刊论文
| 作者 | Lei, Rihua1; Li, Yang1; Cai, Yuerong2; Li, Chenyang2; Pu, Mengna2; Lu, Chengkai1; Yang, Yujie2; Liang, Gang1
|
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2020 |
| 卷号 | 13期号:4页码:634-649 |
| ISSN号 | 1674-2052 |
| 关键词 | bHLH121 bHLH IVc bHLH Ib FIT iron uptake Arabidopsis |
| DOI | 10.1016/j.molp.2020.01.006 |
| 英文摘要 | Iron (Fe) deficiency is prevalent in plants grown in neutral or alkaline soil. Plants have evolved sophisticated mechanisms that regulate Fe homeostasis, ensuring survival. In Arabidopsis, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) is a crucial regulator of Fe-deficiency response. FIT is activated indirectly by basic helix-loop-helix (bHLH) IVc transcription factors (TFs) under Fe deficiency; however, it remains unclear which protein(s) act as the linker to mediate the activation of FIT by bHLH IVc TFs. In this study, we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter. We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms, reduced Fe accumulation, and disrupted expression of genes associated with Fe homeostasis. Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bh1h121 mutant. Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121. We demonstrated that bHLH121 has DNA-binding activity and can bind the promoters of the FIT and bHLH lb genes, but we did not find that it has either direct transcriptional activation or repression activity toward these genes. Meanwhile, we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs, and its expression is induced by Fe deficiency in a bHLH IVc-dependent manner. Taken together, these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000523654200011 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/11654]  |
| 专题 | 西双版纳热带植物园_2012年后新成立研究组 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Mengla 666303, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Rihua,Li, Yang,Cai, Yuerong,et al. bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis[J]. MOLECULAR PLANT,2020,13(4):634-649. |
| APA | Lei, Rihua.,Li, Yang.,Cai, Yuerong.,Li, Chenyang.,Pu, Mengna.,...&Liang, Gang.(2020).bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis.MOLECULAR PLANT,13(4),634-649. |
| MLA | Lei, Rihua,et al."bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis".MOLECULAR PLANT 13.4(2020):634-649. |
入库方式： OAI收割
来源：西双版纳热带植物园
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