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Selective N-glycan editing on living cell surfaces to probe glycoconjugate function

文献类型：期刊论文

作者Tang, Feng1,2,3; Zhou, Mang1; Qin, Ken1,2; Shi, Wei1,2; Yashinov, Ansor1,2; Yang, Yang1; Yang, Liyun1; Guan, Dongliang1; Zhao, Lei1; Tang, Yubo1
刊名NATURE CHEMICAL BIOLOGY
出版日期2020-06-01
页码15
ISSN号1552-4450
DOI10.1038/s41589-020-0551-8
通讯作者Zhou, Mang(mzhou@simm.ac.cn) ; Huang, Wei(huangwei@simm.ac.cn)
英文摘要Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions. Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites. Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions. In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved. Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.
WOS关键词CHEMOENZYMATIC SYNTHESIS ; IGG ANTIBODIES ; ACETYLGLUCOSAMINIDASE ; GLYCOSYLATION ; GLYCOPROTEINS ; MUTANTS ; IDENTIFICATION ; GLYCOPEPTIDES ; STRATEGY ; TRANSGLYCOSYLATION
资助项目National Natural Science Foundation of China[21877116] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program' of China[2018ZX09711002-006] ; China Postdoctoral Science Foundation[BX20180339] ; China Postdoctoral Science Foundation[2018M642120]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000537039100005
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.183/handle/2S10ELR8/280004]  
专题中国科学院上海药物研究所
通讯作者Zhou, Mang; Huang, Wei
作者单位1.Chinese Acad Sci, Ctr Biotherapeut Discovery Res, Shanghai Inst Mat Med, CAS Key Lab Receptor Res,CAS Ctr Excellence Mol C, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou, Peoples R China
4.East China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai, Peoples R China
5.East China Normal Univ, Sch Life Sci, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Feng,Zhou, Mang,Qin, Ken,et al. Selective N-glycan editing on living cell surfaces to probe glycoconjugate function[J]. NATURE CHEMICAL BIOLOGY,2020:15.
APA Tang, Feng.,Zhou, Mang.,Qin, Ken.,Shi, Wei.,Yashinov, Ansor.,...&Huang, Wei.(2020).Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.NATURE CHEMICAL BIOLOGY,15.
MLA Tang, Feng,et al."Selective N-glycan editing on living cell surfaces to probe glycoconjugate function".NATURE CHEMICAL BIOLOGY (2020):15.

入库方式： OAI收割

来源：上海药物研究所

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