Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota
文献类型：期刊论文
| 作者 | Guo, Ciliang1,3; Zhang, Shihai1,3; Wang, Yeqing1,2; Li, Meixia1,3; Ding, Kan1,3
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2020-07-01 |
| 卷号 | 154页码:82-91 |
| 关键词 | Crataegus pinnatifida Polysaccharide Gut microbiota Short chain fatty acid Bacteria competition |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2020.03.058 |
| 通讯作者 | Li, Meixia(limei@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography. HAW1-2 was proved as a homogenous polysaccharide with amolecular weight (Mw) of 8.94 kDa. Monosaccharide composition results indicated that HAW1-2 was composed of arabinose, galactose and glucose. Methylation analysis and NMR spectrum showed that HAW1-2 contained -> 4)-beta-D-Glcp-(1 ->,-> 4)-beta-D-Galp-(1 ->, alpha-L-Araf(1 ->,-> 5)-alpha-L-Araf-(1 ->, beta-D-Glcp-(1 -> and -> 4,6)-beta-D-Glcp-(1 ->. Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp. under co-culture. Interestingly, Bacteroides spp. generated more acetic acids and propionic acids while BL only generated acetic acids. These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids. (C) 2020 Elsevier B.V. All rights reserved. |
| WOS关键词 | CHAIN FATTY-ACIDS ; WATER-SOLUBLE POLYSACCHARIDES ; IMMUNOSTIMULATORY ACTIVITY ; DRIED FRUIT ; HAWTHORN ; BUTYRATE ; RECEPTOR ; FIBER ; DIET ; ARABINOGALACTAN |
| 资助项目 | New Drug Creation and Manufacturing Program[2019ZX09735001] ; Ministry of Science and Technology, the People Republic of China ; National Natural Science Foundation of China[31670814] ; National Natural Science Foundation of China[31870801] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000533508600010 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/280105]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Meixia; Ding, Kan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Nanchang Univ, Sch Pharm, Nanchang 330006, Jiangxi, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Ciliang,Zhang, Shihai,Wang, Yeqing,et al. Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,154:82-91. |
| APA | Guo, Ciliang,Zhang, Shihai,Wang, Yeqing,Li, Meixia,&Ding, Kan.(2020).Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,154,82-91. |
| MLA | Guo, Ciliang,et al."Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 154(2020):82-91. |
入库方式： OAI收割
来源：上海药物研究所
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