中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity

文献类型:期刊论文

作者Zhang, Meng-Qi3,4,5; Ren, Xia3,4; Zhao, Qing3,4; Yue, Shi-Jun3,4; Fu, Xiu-Mei1,3; Li, Xin3,4; Chen, Kai-Xian2,6; Guo, Yue-Wei2,6; Shao, Chang-Lun3,4; Wang, Chang-Yun3,4
刊名JOURNAL OF ETHNOPHARMACOLOGY
出版日期2020-06-28
卷号256页码:8
ISSN号0378-8741
关键词Acanthus ilicifolius L. Total phenylethanoid glycosides Hepatoprotective Antioxidant
DOI10.1016/j.jep.2020.112795
通讯作者Shao, Chang-Lun(shaochanglun@ouc.edu.cn) ; Wang, Chang-Yun(changyun@ouc.edu.cn)
英文摘要Ethnopharmacological relevance: Acanthus ilicifolius L. has been used as a folk medicine in the treatment of acute and chronic hepatitis in China for a long time. Phenylethanoid glycosides are one of main components in A. ilicifolius L. Aim of the study: The aim of present study was to assess the hepatoprotective activities of total phenylethanoid glycosides from A. ilicifolius L. (APhGs) against carbon tetrachloride (CCl4)-induced liver injury in vivo and in vitro. Materials and method: The APhGs was separated by resin column chromatography. The purity of total phenylethanoid glycosides was determined by UV-Vis spectrophotometry using acteoside as a standard. The hepatoprotective activities of APhGs against CCl4-induced liver injury were performed on experimental mice and L-02 hepatocytes. Moreover, the antioxidant activities of APhGs were tested in vitro. Results: The results showed that pre-administration of APhGs to mice decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, and improved superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) level in serum and liver tissue induced by CCl4. Specifically, the SOD activities of APhGs-H and APhGs-M treatment groups were stronger than that of silymarin treatment group. The protective activities of APhGs were confirmed by histopathological results. Moreover, immunohistochemical analysis showed that APhGs could remarkably down-regulate the protein expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-beta (IL-beta). In vitro experiment, APhGs was observed to increase L-02 hepatocyte viability against CCl4-induced hepatotoxicity. In addition, antioxidation assays revealed that APhGs showed 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and ferric reducing ability. Conclusion: Overall, total phenylethanoid glycosides from A. ilicifolius L. displayed promising hepatoprotective effects. These results offer a support for the medicine uses of A. ilicifolius L.
WOS关键词ACUTE LIVER-INJURY ; ANTIOXIDANT ACTIVITY ; ACTEOSIDE ; POLYSACCHARIDES ; EXTRACT ; DAMAGE
资助项目Natural Science Foundation of Shandong Province, China[ZR2019BD049] ; National Natural Science Foundation of China, China[41806191] ; National Science and Technology Major Project for Significant New Drugs Development[2018ZX09735-004] ; Program of Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao, China) ; Taishan Scholars Program, China
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
出版者ELSEVIER IRELAND LTD
WOS记录号WOS:000528279300005
源URL[http://119.78.100.183/handle/2S10ELR8/280172]  
专题新药研究国家重点实验室
通讯作者Shao, Chang-Lun; Wang, Chang-Yun
作者单位1.Ocean Univ China, Coll Econ, Qingdao 266100, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Minist Educ China, Qingdao 266003, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
5.Qilu Univ Technol, Engn Res Ctr Zebrafish Models Human Dis & Drug Sc, Biol Inst, Key Lab Biosensor Shandong Prov,Shandong Acad Sci, Jinan 250103, Peoples R China
6.Pilot Natl Lab Marine Sci & Technol, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Peoples R China
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GB/T 7714
Zhang, Meng-Qi,Ren, Xia,Zhao, Qing,et al. Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity[J]. JOURNAL OF ETHNOPHARMACOLOGY,2020,256:8.
APA Zhang, Meng-Qi.,Ren, Xia.,Zhao, Qing.,Yue, Shi-Jun.,Fu, Xiu-Mei.,...&Wang, Chang-Yun.(2020).Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity.JOURNAL OF ETHNOPHARMACOLOGY,256,8.
MLA Zhang, Meng-Qi,et al."Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity".JOURNAL OF ETHNOPHARMACOLOGY 256(2020):8.

入库方式: OAI收割

来源:上海药物研究所

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