Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity
文献类型:期刊论文
作者 | Zhang, Meng-Qi3,4,5; Ren, Xia3,4; Zhao, Qing3,4; Yue, Shi-Jun3,4; Fu, Xiu-Mei1,3; Li, Xin3,4; Chen, Kai-Xian2,6; Guo, Yue-Wei2,6; Shao, Chang-Lun3,4; Wang, Chang-Yun3,4 |
刊名 | JOURNAL OF ETHNOPHARMACOLOGY |
出版日期 | 2020-06-28 |
卷号 | 256页码:8 |
ISSN号 | 0378-8741 |
关键词 | Acanthus ilicifolius L. Total phenylethanoid glycosides Hepatoprotective Antioxidant |
DOI | 10.1016/j.jep.2020.112795 |
通讯作者 | Shao, Chang-Lun(shaochanglun@ouc.edu.cn) ; Wang, Chang-Yun(changyun@ouc.edu.cn) |
英文摘要 | Ethnopharmacological relevance: Acanthus ilicifolius L. has been used as a folk medicine in the treatment of acute and chronic hepatitis in China for a long time. Phenylethanoid glycosides are one of main components in A. ilicifolius L. Aim of the study: The aim of present study was to assess the hepatoprotective activities of total phenylethanoid glycosides from A. ilicifolius L. (APhGs) against carbon tetrachloride (CCl4)-induced liver injury in vivo and in vitro. Materials and method: The APhGs was separated by resin column chromatography. The purity of total phenylethanoid glycosides was determined by UV-Vis spectrophotometry using acteoside as a standard. The hepatoprotective activities of APhGs against CCl4-induced liver injury were performed on experimental mice and L-02 hepatocytes. Moreover, the antioxidant activities of APhGs were tested in vitro. Results: The results showed that pre-administration of APhGs to mice decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, and improved superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) level in serum and liver tissue induced by CCl4. Specifically, the SOD activities of APhGs-H and APhGs-M treatment groups were stronger than that of silymarin treatment group. The protective activities of APhGs were confirmed by histopathological results. Moreover, immunohistochemical analysis showed that APhGs could remarkably down-regulate the protein expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-beta (IL-beta). In vitro experiment, APhGs was observed to increase L-02 hepatocyte viability against CCl4-induced hepatotoxicity. In addition, antioxidation assays revealed that APhGs showed 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and ferric reducing ability. Conclusion: Overall, total phenylethanoid glycosides from A. ilicifolius L. displayed promising hepatoprotective effects. These results offer a support for the medicine uses of A. ilicifolius L. |
WOS关键词 | ACUTE LIVER-INJURY ; ANTIOXIDANT ACTIVITY ; ACTEOSIDE ; POLYSACCHARIDES ; EXTRACT ; DAMAGE |
资助项目 | Natural Science Foundation of Shandong Province, China[ZR2019BD049] ; National Natural Science Foundation of China, China[41806191] ; National Science and Technology Major Project for Significant New Drugs Development[2018ZX09735-004] ; Program of Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao, China) ; Taishan Scholars Program, China |
WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
语种 | 英语 |
出版者 | ELSEVIER IRELAND LTD |
WOS记录号 | WOS:000528279300005 |
源URL | [http://119.78.100.183/handle/2S10ELR8/280172] |
专题 | 新药研究国家重点实验室 |
通讯作者 | Shao, Chang-Lun; Wang, Chang-Yun |
作者单位 | 1.Ocean Univ China, Coll Econ, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Minist Educ China, Qingdao 266003, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 5.Qilu Univ Technol, Engn Res Ctr Zebrafish Models Human Dis & Drug Sc, Biol Inst, Key Lab Biosensor Shandong Prov,Shandong Acad Sci, Jinan 250103, Peoples R China 6.Pilot Natl Lab Marine Sci & Technol, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Meng-Qi,Ren, Xia,Zhao, Qing,et al. Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity[J]. JOURNAL OF ETHNOPHARMACOLOGY,2020,256:8. |
APA | Zhang, Meng-Qi.,Ren, Xia.,Zhao, Qing.,Yue, Shi-Jun.,Fu, Xiu-Mei.,...&Wang, Chang-Yun.(2020).Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity.JOURNAL OF ETHNOPHARMACOLOGY,256,8. |
MLA | Zhang, Meng-Qi,et al."Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity".JOURNAL OF ETHNOPHARMACOLOGY 256(2020):8. |
入库方式: OAI收割
来源:上海药物研究所
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