Synthetic antibodies against BRIL as universal fiducial marks for single?particle cryoEM structure determination of membrane proteins
文献类型：期刊论文
| 作者 | Somnath Mukherjee; Satchal K. Erramilli; Mark Ammirati; Frances J. D. Alvarez; Kimberly F. Fennell; Michael D. Purdy; Blazej M. Skrobek; Katarzyna Radziwon; John Coukos; Yanyong Kang |
| 刊名 | naturecommunications
 |
| 出版日期 | 2020 |
| 卷号 | 11期号:1页码:1 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-020-15363-0 |
| 英文摘要 | We propose the concept of universal fiducials based on a set of pre-made semi-synthetic antibodies (sABs)generated by customized phage display selections against the fusion protein BRIL, an engineered variant of apocytochrome b562a. These sABs can bind to BRIL fused either into the loops or termini of different GPCRs, ion channels, receptors and transporters without disrupting their structure. A crystal structure of BRIL in complex with an affinity-matured sAB (BAG2) that bound to all systems tested delineates the footprint of interaction. Negative stain and cryoEM data of several examples of BRIL-membrane protein chimera highlight the effectiveness of the sABs as universal fiducial marks. Taken together with a cryoEM structure of sAB bound human nicotinic acetylcholine receptor, this work demonstrates that these anti-BRIL sABs can greatly enhance the particle properties leading to improved cryoEM outcomes, especially for challenging membrane proteins. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/280404]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.University of Wisconsin Madison 2.The University of Chicago 3.Medicine Design, Worldwide Research and Development, Pfizer Inc. 4.University of Virginia School of Medicine 5.Center for Cancer and Cell Biology, Structural Biology Program, Van Andel Research Institute 6.Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences 7.University of Chicago 8.Ludwig Maximilian University of Munich 9.Takeda San Diego Inc. 10.California Institute of Technology |
| 推荐引用方式 GB/T 7714 | Somnath Mukherjee,Satchal K. Erramilli,Mark Ammirati,et al. Synthetic antibodies against BRIL as universal fiducial marks for single?particle cryoEM structure determination of membrane proteins[J]. naturecommunications,2020,11(1):1. |
| APA | Somnath Mukherjee.,Satchal K. Erramilli.,Mark Ammirati.,Frances J. D. Alvarez.,Kimberly F. Fennell.,...&Anthony A. Kossiakoff.(2020).Synthetic antibodies against BRIL as universal fiducial marks for single?particle cryoEM structure determination of membrane proteins.naturecommunications,11(1),1. |
| MLA | Somnath Mukherjee,et al."Synthetic antibodies against BRIL as universal fiducial marks for single?particle cryoEM structure determination of membrane proteins".naturecommunications 11.1(2020):1. |
入库方式： OAI收割
来源：上海药物研究所
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