中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry

文献类型：期刊论文

作者Zheng, Xiaoli1,3; Duan, Haonan2,3,4; Lin, Feifei3; Li, Xiaomei3,4; Shen, Jianhua2,3,4; Han, Fanghui3,4; Huang, Fubao3,4; Li, Shilin3; Chang, Lu3; Xu, Hui1
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2020-05-01
卷号412期号:13页码:3241-3252
ISSN号1618-2642
关键词Phenols Aromatic acid Microbiota Uremic toxin Simultaneous derivatization 2-Bromo-1-[4-(dimethylamino)phenyl] ethyl ketone
DOI10.1007/s00216-020-02585-w
通讯作者Xu, Hui(xuhui@ytu.edu.cn) ; Wang, Kai(kwang@simm.ac.cn) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要In the intestine, several phenols and aromatic acids are generated by microbiota and are highly related to the formation of uremic toxins. Herein, we developed a new derivatization reagent, 2-bromo-1-[4-(dimethylamino)phenyl] ethyl ketone (BDAPE), that reacted simultaneously with phenols and aromatic acids. Following a reaction within 2 h at 60 degrees C in the presence of 200 mM potassium carbonate (K2CO3), the obtained BDAPE derivatives were separated on a reversed-phase C18 column and quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in positive electrospray ionization mode. This method allowed a lower limit of quantification (LLOQ) of 0.090 mu M for 3-indolepropionic acid (3IPA), indole-3-acetic acid (3IAA), p-cresol (PC), benzoic acid (BA), and phenol (PN); 0.30 mu M for phenylacetic acid (PAA); 0.45 mu M for 4-hydroxyphenylacetic acid (4HPAA); and 0.60 mu M for 3-phenylpropionic acid (PPA). Methodological validation further demonstrated acceptable accuracy (%RE < 16.1) and precision (%RSD < 16.2), suggesting that this is a sensitive and robust method for simultaneous quantification of phenols and aromatic acids. The method was successfully applied to analyze these microbiota-related analytes in mouse feces of a diabetic nephropathy model. Graphical abstract
WOS关键词CHRONIC KIDNEY-DISEASE ; FATTY-ACIDS ; URINE
资助项目Youth Innovation Promotion Association, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica (SIMM), Chinese Academy of Sciences ; National Natural Science Foundation of China[81803610] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050306] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050408] ; National Science and Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002-006-014] ; Key Research and Development Project of Shandong Province[2018GSF119010] ; Natural Science Foundation of Shandong Province[ZR2017MH061]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000529114600001
源URL[http://119.78.100.183/handle/2S10ELR8/280440]  
专题新药研究国家重点实验室
通讯作者Xu, Hui; Wang, Kai; Liu, Jia
作者单位1.Yantai Univ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ,Collaborat Innovat Ctr Adv Drug Deliv, Yantai 264005, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Xiaoli,Duan, Haonan,Lin, Feifei,et al. Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020,412(13):3241-3252.
APA Zheng, Xiaoli.,Duan, Haonan.,Lin, Feifei.,Li, Xiaomei.,Shen, Jianhua.,...&Liu, Jia.(2020).Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412(13),3241-3252.
MLA Zheng, Xiaoli,et al."Quantification of microbiota-related phenols and aromatic acids in mouse feces of a diabetic nephropathy model by simultaneous BDAPE derivatization using ultra-performance liquid chromatography-tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412.13(2020):3241-3252.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。