Highly selective monitoring of in-source fragmentation sapogenin product ions in positive mode enabling group-target ginsenosides profiling and simultaneous identification of seven Panax herbal medicines
文献类型：期刊论文
| 作者 | Zhang, Chun-xia3,4; Wang, Xiao-yan3,4; Lin, Zhao-zhou1; Wang, Hong-da3,4; Qian, Yue-xin3,4; Li, Wei-wei3,4; Yang, Wen-zhi3,4; Guo, De-an2,3,4
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
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| 出版日期 | 2020-05-10 |
| 卷号 | 1618页码:11 |
| 关键词 | In-source fragmentation Ginsenoside Selective ion monitoring Characteristic chromatogram Panax Traditional Chinese medicine formula |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2020.460850 |
| 通讯作者 | Yang, Wen-zhi(wzyang0504@tjutcm.edu.cn) ; Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | In-source fragmentation of ginsenosides in the positive ESI mode (pISF-G) frequently occurs, which results in little fragment information useful for the structural elucidation. We are aimed to unveil the genesic mechanism and explore its potential significance in quality control of Ginseng and the related compound formulae. By applying six high-resolution mass spectrometers from Agilent, Waters, and Thermo Fisher, we could primarily demonstrate the susceptibility of pISF-G. The ion clusters in the positive fullscan MS1 spectra were generated from the protonated sapogenins by successive elimination of H2O, and showed specificity for ginsenoside classification. Selective ion monitoring (SIM) of the sapogenin product ions could delineate group-target ginsenoside profiles from Ginseng. A high-selectivity characteristic chromatogram (CC) was elaborated for Ginseng, on the VionTM IMS-QTOF mass spectrometer by IM (ion mobility) separation and quadrupole filtering of four sapogenin fragments (m/z 407.37/CCS 206.24 angstrom(2); m/z 423.36/CCS 211.26 angstrom(2); m/z 439.36/CCS 209.60 angstrom(2); m/z 457.37/CCS 217.81 angstrom(2)). Chemometricanalysis, based on the CC data of seven Ginseng drugs (P. ginseng, P. quinquefolius, P. notoginseng, Red ginseng, leaf of P. ginseng, P. japonicus, and P. japonicus var. major), disclosed 35 marker compounds. We could readily discriminate among P. ginseng, P. quinquefolius, and P. notoginseng, in 15 different compound formulae by identifying these marker compounds on both the Vion IMS-QTOF and QTrap 4500 mass spectrometers. Conclusively, SIM of the pISF-G sapogenin product ions renders a new concept of CC enabling the group-target profiling of ginsenosides and authentication of Ginseng and the related compound formulae. (C) 2020 Elsevier B.V. All rights reserved. |
| WOS关键词 | LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; GINSENG SAPONINS ; METABOLOMICS ; TRAP ; DIFFERENTIATION ; STRATEGY ; QUINQUEFOLIUS ; NOTOGINSENG ; RESOLUTION |
| 资助项目 | National Natural Science Foundation of China[81872996] ; State Key Research and Development Project-Special Research of TCM modernization[2017YFC1702104] ; State Key Research and Development Project-Special Research of TCM modernization[2018YFC1707904] ; State Key Research and Development Project-Special Research of TCM modernization[2018YFC1707905] ; State Key Project for the Creation of Major New Drugs[2018ZX09711001-009-010] ; State Key Project for the Creation of Major New Drugs[2018ZX09735-002] ; Tianjin Municipal Education Commission Research Project[2017ZD07] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000528932300027 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/280530]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Wen-zhi; Guo, De-an |
| 作者单位 | 1.Beijing Inst Chinese Med, Beijing 100010, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, 501 Haike Rd, Shanghai 201203, Peoples R China 3.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, 312 Anshanxi Rd, Tianjin 300193, Peoples R China 4.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, 312 Anshanxi Rd, Tianjin 300193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chun-xia,Wang, Xiao-yan,Lin, Zhao-zhou,et al. Highly selective monitoring of in-source fragmentation sapogenin product ions in positive mode enabling group-target ginsenosides profiling and simultaneous identification of seven Panax herbal medicines[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1618:11. |
| APA | Zhang, Chun-xia.,Wang, Xiao-yan.,Lin, Zhao-zhou.,Wang, Hong-da.,Qian, Yue-xin.,...&Guo, De-an.(2020).Highly selective monitoring of in-source fragmentation sapogenin product ions in positive mode enabling group-target ginsenosides profiling and simultaneous identification of seven Panax herbal medicines.JOURNAL OF CHROMATOGRAPHY A,1618,11. |
| MLA | Zhang, Chun-xia,et al."Highly selective monitoring of in-source fragmentation sapogenin product ions in positive mode enabling group-target ginsenosides profiling and simultaneous identification of seven Panax herbal medicines".JOURNAL OF CHROMATOGRAPHY A 1618(2020):11. |
入库方式： OAI收割
来源：上海药物研究所
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