中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry

文献类型:期刊论文

作者Fu, Huan1,3; Zhang, Qing-li3; Huang, Xiao-wu2; Ma, Zheng-hua3; Zheng, Xiao-li3; Li, Shi-lin3; Duan, Hao-nan3; Sun, Xiao-chu1; Lin, Fei-fei3; Zhao, Li-juan3
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
出版日期2020-05-15
卷号34期号:9页码:11
ISSN号0951-4198
DOI10.1002/rcm.8730
通讯作者Teng, Guo-sheng(gshteng@163.com) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要Rationale Short-chain fatty acids (SCFAs) are associated with intestinal microbiota and diseases in humans. SCFAs have a low response in mass spectrometry, and in order to increase sensitivity, reduce sample consumption, shorten analysis time, and simplify sample preparation steps, a derivatization method was developed. Methods We converted seven SCFAs into amide derivatives with 4-aminomethylquinoline. The reaction occurred for 20 min at room temperature. The analytes were separated on a reversed-phase C18 column and quantitated in the positive ion electrospray ionization mode using multiple reaction monitoring. Acetic acid-d(4) was used as the stable-isotope-labeled surrogate analyte for acetic acid in the working solutions, while the other stable-isotope-labeled standards were used as internal standards (ISs). Results Method validation showed that the intra-day and inter-day precision of quantitation for the seven SCFAs over the whole concentration range was <= 3.8% (n = 6). The quantitation accuracy ranged from 85.5% to 104.3% (n = 6). Most important, the collected feces were vortexed immediately with ethanol. Conclusions This study provides a new derivatization method for a precise, accurate, and rapid quantitation of SCFAs in human feces using ultra-performance liquid chromatography/tandem mass spectrometry. This method successfully determined the concentration of SCFAs in human feces and could assist in the exploration of intestinal microbiota and diseases.
WOS关键词QUANTIFICATION ; METABOLITES ; BIOMARKERS
资助项目National Natural Science Foundation of China[81803610] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
出版者WILEY
WOS记录号WOS:000529870500012
源URL[http://119.78.100.183/handle/2S10ELR8/280574]  
专题中国科学院上海药物研究所
通讯作者Teng, Guo-sheng; Liu, Jia
作者单位1.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Peoples R China
2.PLA Army Gen Hosp, Beijing, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式
GB/T 7714
Fu, Huan,Zhang, Qing-li,Huang, Xiao-wu,et al. A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2020,34(9):11.
APA Fu, Huan.,Zhang, Qing-li.,Huang, Xiao-wu.,Ma, Zheng-hua.,Zheng, Xiao-li.,...&Liu, Jia.(2020).A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,34(9),11.
MLA Fu, Huan,et al."A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 34.9(2020):11.

入库方式: OAI收割

来源:上海药物研究所

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