A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry
文献类型:期刊论文
作者 | Fu, Huan1,3; Zhang, Qing-li3; Huang, Xiao-wu2; Ma, Zheng-hua3; Zheng, Xiao-li3; Li, Shi-lin3; Duan, Hao-nan3; Sun, Xiao-chu1; Lin, Fei-fei3; Zhao, Li-juan3 |
刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY |
出版日期 | 2020-05-15 |
卷号 | 34期号:9页码:11 |
ISSN号 | 0951-4198 |
DOI | 10.1002/rcm.8730 |
通讯作者 | Teng, Guo-sheng(gshteng@163.com) ; Liu, Jia(jia.liu@simm.ac.cn) |
英文摘要 | Rationale Short-chain fatty acids (SCFAs) are associated with intestinal microbiota and diseases in humans. SCFAs have a low response in mass spectrometry, and in order to increase sensitivity, reduce sample consumption, shorten analysis time, and simplify sample preparation steps, a derivatization method was developed. Methods We converted seven SCFAs into amide derivatives with 4-aminomethylquinoline. The reaction occurred for 20 min at room temperature. The analytes were separated on a reversed-phase C18 column and quantitated in the positive ion electrospray ionization mode using multiple reaction monitoring. Acetic acid-d(4) was used as the stable-isotope-labeled surrogate analyte for acetic acid in the working solutions, while the other stable-isotope-labeled standards were used as internal standards (ISs). Results Method validation showed that the intra-day and inter-day precision of quantitation for the seven SCFAs over the whole concentration range was <= 3.8% (n = 6). The quantitation accuracy ranged from 85.5% to 104.3% (n = 6). Most important, the collected feces were vortexed immediately with ethanol. Conclusions This study provides a new derivatization method for a precise, accurate, and rapid quantitation of SCFAs in human feces using ultra-performance liquid chromatography/tandem mass spectrometry. This method successfully determined the concentration of SCFAs in human feces and could assist in the exploration of intestinal microbiota and diseases. |
WOS关键词 | QUANTIFICATION ; METABOLITES ; BIOMARKERS |
资助项目 | National Natural Science Foundation of China[81803610] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000529870500012 |
源URL | [http://119.78.100.183/handle/2S10ELR8/280574] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Teng, Guo-sheng; Liu, Jia |
作者单位 | 1.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Peoples R China 2.PLA Army Gen Hosp, Beijing, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Fu, Huan,Zhang, Qing-li,Huang, Xiao-wu,et al. A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2020,34(9):11. |
APA | Fu, Huan.,Zhang, Qing-li.,Huang, Xiao-wu.,Ma, Zheng-hua.,Zheng, Xiao-li.,...&Liu, Jia.(2020).A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,34(9),11. |
MLA | Fu, Huan,et al."A rapid and convenient derivatization method for quantitation of short-chain fatty acids in human feces by ultra-performance liquid chromatography/tandem mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 34.9(2020):11. |
入库方式: OAI收割
来源:上海药物研究所
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