中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus

文献类型：期刊论文

作者Yang, Nana1,2; Cao, Qiao2,5; Hu, Shuyang1,2; Xu, Chenchen1,2; Fan, Ke1,2; Chen, Feifei2,5; Yang, Cai-Guang1,2; Liang, Haihua5; Wu, Min6; Bae, Taeok4
刊名MOLECULAR MICROBIOLOGY
出版日期2020-05-08
页码20
关键词ClpXP microbial interspecies interactions proteolysis Pseudomonas aeruginosa quorum sensing
ISSN号0950-382X
DOI10.1111/mmi.14519
通讯作者Lan, Lefu(llan@simm.ac.cn)
英文摘要Intracellular protein degradation is essential for the survival of all organisms, but its role in interspecies interaction is unknown. Here, we show that the ClpXP protease of Pseudomonas aeruginosa suppresses its antimicrobial activity against Staphylococcus aureus, a common pathogen co-isolated with P. aeruginosa from polymicrobial human infections. Using proteomic, biochemical, and molecular genetic approaches, we found that this effect is due to the inhibitory effects of ClpXP on the quorum sensing (QS) of P. aeruginosa, mainly by degrading proteins (e.g., PhnA, PhnB, PqsR, and RhlI) which are critical for the production of QS signal molecules PQS and C4-HSL. We provide evidence that co-culturing with S. aureus induces a decrease in the activity of ClpXP in P. aeruginosa, an effect which was also achieved by the treatment of P. aeruginosa with N-acetylglucosamine (GlcNAc), a widespread chemical present on the surface of diverse cell types from bacteria to humans. These findings extend the range of biological events governed by proteolytic machinery to microbial community structure, thus also suggesting that a chemical-induced alteration of protein homeostasis is a mechanism for interspecies interactions.
WOS关键词HOST-RANGE PLASMIDS ; REGULATED PROTEOLYSIS ; VIRULENCE FACTORS ; GENE-EXPRESSION ; CLPP ; BACTERIA ; TOLERANCE ; PROTEASES ; REVEALS ; RHLR
资助项目National Natural Science Foundation of China[31670136] ; National Natural Science Foundation of China[31870127] ; National Natural Science Foundation of China[81861138047] ; National Natural Science Foundation of China[81661138004] ; Ministry of Science and Technology of the People's Republic of China[2016YFA0501503] ; State Key Laboratory of Drug Research[SIMM2003ZZ-03] ; Science and Technology Commission of Shanghai Municipality[19JC1416400]
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
语种英语
WOS记录号WOS:000530928200001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/280607]  
专题新药研究国家重点实验室
通讯作者Lan, Lefu
作者单位1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
3.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai, Peoples R China
4.Indiana Univ, Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN USA
5.Northwest Univ, Coll Life Sci, Xian, Peoples R China
6.Univ North Dakota, Dept Biomed Sci, Grand Forks, ND USA
推荐引用方式
GB/T 7714		 Yang, Nana,Cao, Qiao,Hu, Shuyang,et al. Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus[J]. MOLECULAR MICROBIOLOGY,2020:20.
APA Yang, Nana.,Cao, Qiao.,Hu, Shuyang.,Xu, Chenchen.,Fan, Ke.,...&Lan, Lefu.(2020).Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus.MOLECULAR MICROBIOLOGY,20.
MLA Yang, Nana,et al."Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus".MOLECULAR MICROBIOLOGY (2020):20.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。