acomplexstructureofarrestin2boundtoagproteincoupledreceptor
文献类型：期刊论文
| 作者 | Yin Wanchao2; Li Zhihai2; Jin Mingliang3; Yin Yuling2; De Waal Parker W4; Pal Kuntal4; Yin Yanting2; Gao Xiang4; He Yuanzheng4; Gao Jing2 |
| 刊名 | cellresearch
 |
| 出版日期 | 2019 |
| 卷号 | 29期号:12页码:971 |
| ISSN号 | 1001-0602 |
| 关键词 | NEUROTENSIN VISUALIZATION RECRUITMENT AGONIST ML314 |
| DOI | 10.1038/s41422-019-0256-2 |
| 英文摘要 | Arrestins comprise a family of signal regulators of G-protein-coupled receptors (GPCRs), which include arrestins 1 to 4. While arrestins 1 and 4 are visual arrestins dedicated to rhodopsin, arrestins 2 and 3 (Arr2 and Arr3) are beta-arrestins known to regulate many nonvisual GPCRs. The dynamic and promiscuous coupling of Arr2 to nonvisual GPCRs has posed technical challenges to tackle the basis of arrestin binding to GPCRs. Here we report the structure of Arr2 in complex with neurotensin receptor 1 (NTSR1), which reveals an overall assembly that is strikingly different from the visual arrestin-rhodopsin complex by a 90 degrees rotation of Arr2 relative to the receptor. In this new configuration, intracellular loop 3 (ICL3) and transmembrane helix 6 (TM6) of the receptor are oriented toward the N-terminal domain of the arrestin, making it possible for GPCRs that lack the C-terminal tail to couple Arr2 through their ICL3. Molecular dynamics simulation and crosslinking data further support the assembly of the Arr2.NTSR1 complex. Sequence analysis and homology modeling suggest that the Arr2. NTSR1 complex structure may provide an alternative template for modeling arrestin-GPCR interactions. |
| 资助项目 | [Ministry of Science and Technology (China)] ; [Van Andel Research Institute] ; [National Institutes of Health grants] ; [100 Talents Program of the Chinese Academy of Sciences] ; [Chinese Academy of Sciences grant] ; [Natural Science Foundation of Shanghai] ; [Shanghai Sailing Program] ; [China Postdoctoral Science Foundation] ; [National Basic Research Program of China] ; [NSFC] ; [CAS] ; [CAS Major Science and Technology Infrastructure Open Research Projects] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/280665]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.浙江大学医学部 2.中国科学院上海药物研究所 3.中国科学院大学 4.Center for Cancer and Cell Biology, Program for Structural Biology, Van Andel Research Institute |
| 推荐引用方式 GB/T 7714 | Yin Wanchao,Li Zhihai,Jin Mingliang,et al. acomplexstructureofarrestin2boundtoagproteincoupledreceptor[J]. cellresearch,2019,29(12):971. |
| APA | Yin Wanchao.,Li Zhihai.,Jin Mingliang.,Yin Yuling.,De Waal Parker W.,...&Eric Xu H.(2019).acomplexstructureofarrestin2boundtoagproteincoupledreceptor.cellresearch,29(12),971. |
| MLA | Yin Wanchao,et al."acomplexstructureofarrestin2boundtoagproteincoupledreceptor".cellresearch 29.12(2019):971. |
入库方式： OAI收割
来源：上海药物研究所
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