Spatial filter and its application in three-dimensional single molecule localization microscopy
文献类型：期刊论文
| 作者 | Fan, Xiaoming2,3,4; Hendriks, Johnny2; Comini, Maddalena1,2; Katranidis, Alexandros3; Bueldt, Georg3,5; Gensch, Thomas2 |
| 刊名 | METHODS AND APPLICATIONS IN FLUORESCENCE
 |
| 出版日期 | 2020-04-01 |
| 卷号 | 8期号:2页码:12 |
| 关键词 | three-dimensional imaging fluorescence dSTORM PALM vesicles mitochondria actin filaments |
| ISSN号 | 2050-6120 |
| DOI | 10.1088/2050-6120/ab7e0f |
| 通讯作者 | Fan, Xiaoming(xiaomingfan2015@sina.com) ; Gensch, Thomas(t.gensch@fz-juelich.de) |
| 英文摘要 | Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to determine the axial positions of fluorescent molecules and nanoparticles on the nanometer scale by the usage of two spatial filters, which are placed in two otherwise identical emission detection channels. SFSMLM allows axial localization in a range of ca. 1.5 mu m with a localization precision of 15 - 30 nm in axial direction. The technique was utilized for localizing and imaging small cellular structures - e.g. actin filaments, vesicles and mitochondria - in three dimensions. |
| WOS关键词 | OPTICAL RECONSTRUCTION MICROSCOPY ; SUPERRESOLUTION MICROSCOPY ; FLUORESCENCE MICROSCOPY ; DIFFRACTION-LIMIT ; ACTIN ; DYNAMICS ; TRACKING ; REVEALS |
| 资助项目 | Deutscher Akademischer Austausch Dienst (DAAD) |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000521503100001 |
| 出版者 | IOP PUBLISHING LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/280977]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Fan, Xiaoming; Gensch, Thomas |
| 作者单位 | 1.Univ Oxford, Dept Physiol Anatomy& Genet, Oxford, England 2.Forschungszentrum Julich, Cellular Biophys, Inst Complex Syst ICS 4, Leo Brandt Str, D-52428 Julich, Germany 3.Forschungszentrum Julich, Mol Biophys, Inst Complex Syst ICS 5, Leo Brandt Str, D-52428 Julich, Germany 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Moscow Inst Phys & Technol, Lab Adv Studies Membrane Prot, Dolgoprudnyi, Russia |
| 推荐引用方式 GB/T 7714 | Fan, Xiaoming,Hendriks, Johnny,Comini, Maddalena,et al. Spatial filter and its application in three-dimensional single molecule localization microscopy[J]. METHODS AND APPLICATIONS IN FLUORESCENCE,2020,8(2):12. |
| APA | Fan, Xiaoming,Hendriks, Johnny,Comini, Maddalena,Katranidis, Alexandros,Bueldt, Georg,&Gensch, Thomas.(2020).Spatial filter and its application in three-dimensional single molecule localization microscopy.METHODS AND APPLICATIONS IN FLUORESCENCE,8(2),12. |
| MLA | Fan, Xiaoming,et al."Spatial filter and its application in three-dimensional single molecule localization microscopy".METHODS AND APPLICATIONS IN FLUORESCENCE 8.2(2020):12. |
入库方式： OAI收割
来源：上海药物研究所
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