Heme protects intestinal mucosal barrier in DSS-induced colitis through regulating macrophage polarization in both HO-1-dependent and HO-1-independent way
文献类型：期刊论文
| 作者 | Wu, Yanwei1; Wu, Bing1,2; Zhang, Zongwang3; Lu, Huimin1,2; Fan, Chen1; Qi, Qing1; Gao, Yuanzhuo1,2; Li, Heng1,2; Feng, Chunlan1; Zuo, Jianping2,4
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| 刊名 | FASEB JOURNAL
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| 出版日期 | 2020-04-17 |
| 页码 | 16 |
| ISSN号 | 0892-6638 |
| 关键词 | IBD M1 M2 ulcerative colitis |
| DOI | 10.1096/fj.202000313RR |
| 通讯作者 | Zuo, Jianping(jpzuo@simm.ac.cn) ; Tang, Wei(tangwei@simm.ac.cn) |
| 英文摘要 | Hemoglobin-derived heme was reported to play protective roles in hemorrhagic diseases by modulating the macrophages toward recovery. Mucosal bleeding is one of the pathological features of inflammatory bowel diseases (IBD). However, whether heme provides anti-inflammatory profiles in macrophages, thus contributing to the intestinal mucosal barrier protection, is unclear. In the current study, we investigated the beneficial effects of heme on DSS-induced colitis mice and explored the underlying mechanisms. In vivo, systemic heme supplementation by hemin injection relieved intestinal inflammation and remedied intestinal mucosal barrier damage by correcting abnormal intestinal macrophage polarization. In vitro, we confirmed the reciprocally regulating effects of hemin on M1/M2 macrophage polarization in BMDM. Intriguingly, with knockdown of HO-1, the inhibiting effects of hemin on M1 polarization were maintained, while the promoting effects on M2 polarization were reversed. Further research proved that hemin repressed the inflammatory profiles in macrophages through inhibiting the translocation of NF-kappa B p65 by disrupting IRF5-NF-kappa B p65 complex formation in Spi-C-dependent way. In conclusion, these results showed that the modification of colon tissue microenvironment with heme supplementation plays a protective role in DSS-induced colitis mice through regulating the macrophage polarization in both HO-1-dependent and HO-1-independent way, indicating a new choice to therapeutically modulate the macrophage function and prevent IBD. |
| WOS关键词 | INFLAMMATORY-BOWEL-DISEASE ; INDUCTION ; RECOVERY ; SUPPRESSION ; EXPRESSION ; RESPONSES ; MONOCYTE ; TARGETS ; CELLS |
| 资助项目 | National Natural Science Foundation of China (NSFC)[81673445] ; National Natural Science Foundation of China (NSFC)[81803543] ; Shanghai Committee of Science and Technology[16431900300] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020107] ; National Science & Technology Major Project[2017ZX09101002002-010] ; Chinese Academy of Sciences[X2017ZX09101002-002-010] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000526645200001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281044]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zuo, Jianping; Tang, Wei |
| 作者单位 | 1.Chinese Acad Sci, Lab Antiinflammat, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, Beijing, Peoples R China 3.Shanghai Univ, Sch Life Sci, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, State Key Lab Drug Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yanwei,Wu, Bing,Zhang, Zongwang,et al. Heme protects intestinal mucosal barrier in DSS-induced colitis through regulating macrophage polarization in both HO-1-dependent and HO-1-independent way[J]. FASEB JOURNAL,2020:16. |
| APA | Wu, Yanwei.,Wu, Bing.,Zhang, Zongwang.,Lu, Huimin.,Fan, Chen.,...&Tang, Wei.(2020).Heme protects intestinal mucosal barrier in DSS-induced colitis through regulating macrophage polarization in both HO-1-dependent and HO-1-independent way.FASEB JOURNAL,16. |
| MLA | Wu, Yanwei,et al."Heme protects intestinal mucosal barrier in DSS-induced colitis through regulating macrophage polarization in both HO-1-dependent and HO-1-independent way".FASEB JOURNAL (2020):16. |
入库方式： OAI收割
来源：上海药物研究所
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