Comparative evaluation of label-free quantification strategies
文献类型：期刊论文
| 作者 | Zhao, Lei2; Cong, Xiaoji1,3; Zhai, Linhui1; Hu, Hao1; Xu, Jun-Yu1; Zhao, Wensi1,3; Zhu, Mengdi1,3; Tan, Minjia1,3 ; Ye, Bang-Ce2
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| 刊名 | JOURNAL OF PROTEOMICS
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| 出版日期 | 2020-03-20 |
| 卷号 | 215页码:8 |
| ISSN号 | 1874-3919 |
| 关键词 | Label-free quantification Proteomics Precision Accuracy |
| DOI | 10.1016/j.jprot.2020.103669 |
| 通讯作者 | Tan, Minjia(mjtan@simm.ac.cn) ; Ye, Bang-Ce(bcye@ecust.edu.cn) |
| 英文摘要 | The selection of a data processing method for use in mass spectrometry-based label-free proteome quantification contributes significantly to its accuracy and precision. In this study, we comprehensively evaluated 7 commonly-used label-free quantification methods (MaxQuant-Spectrum count, MaxQuant-iBAQ, MaxQuant-LFQ, MaxQuant-LFAQ, Proteome Discoverer, MetaMorpheus, TPP-StPeter) with a focus on missing values, precision, accuracy, selectivity, and reproducibility of low abundance protein quantification in both single shot and fractionation. Our results showed that among the tested strategies, MaxQuant in MaxLFQ mode outperformed other strategies in terms of accuracy and precision in both whole proteome and low abundance proteome quantification, whereas the Proteome Discoverer (PD) strategy using SEQUEST as a search engine performed better in terms of quantifiable low abundance proteome coverage. We subsequently applied the PD and MaxLFQ strategies in a blood proteomic dataset and found that many FDA-approved tumor prognostic biomarkers could be identified as well as quantified using the PD strategy, indicating the potential advantage of PD in label-free quantification studies. These results provide a reference for method choice in label-free quantification data analysis. Significance: Mass spectrometry-based label-free quantification methods play an important role in label-free proteome data analysis. In this study, we evaluated 7 commonly-used label-free quantification methods with respect to the following aspects: missing values, precision, accuracy, selectivity, and reproducibility for low abundance protein quantification. The results showed that, among the strategies evaluated, the PD strategy with SEQUEST as a search engine performed better in terms of low abundance protein coverage. This study provides a reference for method choice in label-free quantification data analysis. |
| WOS关键词 | POLYMERIC IMMUNOGLOBULIN RECEPTOR ; COMPLEX PROTEIN MIXTURES ; MASS-SPECTROMETRY ; PLASMA PROTEOME |
| 资助项目 | Special Project on Precision Medicine under the National Key RD Program[2017YFC0906600] ; National Natural Science Foundation of China[31730004] ; National Natural Science Foundation of China[21575089] ; National Natural Science Foundation of China[31670066] ; National Natural Science Foundation of China[91753203] ; Natural Science Foundation of China for Innovation Research Group[81821005] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000519667800012 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281120]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Tan, Minjia; Ye, Bang-Ce |
| 作者单位 | 1.Chinese Acad Sci, Chem Prote Ctr, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 2.East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lei,Cong, Xiaoji,Zhai, Linhui,et al. Comparative evaluation of label-free quantification strategies[J]. JOURNAL OF PROTEOMICS,2020,215:8. |
| APA | Zhao, Lei.,Cong, Xiaoji.,Zhai, Linhui.,Hu, Hao.,Xu, Jun-Yu.,...&Ye, Bang-Ce.(2020).Comparative evaluation of label-free quantification strategies.JOURNAL OF PROTEOMICS,215,8. |
| MLA | Zhao, Lei,et al."Comparative evaluation of label-free quantification strategies".JOURNAL OF PROTEOMICS 215(2020):8. |
入库方式： OAI收割
来源：上海药物研究所
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