Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells
文献类型：期刊论文
| 作者 | Zeng, Hui1,2; Huang, Lulin1,2; Tao, Hong1,2; Zhang, Yiwen1,2; Ding, Kan1,2
|
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2020-05-15 |
| 卷号 | 236页码:9 |
| 关键词 | Polygala tenuifolia Pectin Neuritogenesis Neurodegenerative disorders |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2020.116048 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | Pectin is a family of heteropolysaccharides and highly valued for its various bioactivities. Here, a pectin, RP01-1, was purified from roots of Polygala tenuifolia with the molecular weight of 79.1 kDa. Its structure was characterized as alternate 1, 2, 4-linked alpha-Rhap and 1, 4-linked alpha-GalpA constituted the backbone, with branches of terminal (T) -, 1, 4- and 1, 3, 6-linked beta-Galp, T-, 1, 5- and 1, 3, 5-linked alpha-Araf substituted at C-4 of 1, 2, 4-linked alpha-Rhap. The fluorescence spectroscopic analysis indicated that RP01-1 induced neurite outgrowth in PC12 cells and primary cortex neurons in dose dependent manner with no significant cytotoxicity. To disclose the mechanism of this phenomenon, we found that RP01-1 might induce neuritogenesis in PC12 cells via increasing BDNF expression, and promoting the phosphorylation of AKT, ERK, CREB. The results suggest that AKT, ERK, CREB related signaling pathways might implicate in neuritogenesis-inducing activity of RP01-1. |
| WOS关键词 | MOUSE HIPPOCAMPUS ; LONICERA-JAPONICA ; GROWTH ; DIFFERENTIATION ; POLYSACCHARIDE ; NEUROGENESIS ; ACTIVATION ; OUTGROWTH ; ADULT ; CREB |
| 资助项目 | New Drug Creation and Manufacturing Program, Ministry of Science and Technology, the People Republic of China[2019ZX09735001] ; National Natural Science Foundation of China[31670814] ; National Natural Science Foundation of China[31870801] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000519306900065 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281155]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Hui,Huang, Lulin,Tao, Hong,et al. Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells[J]. CARBOHYDRATE POLYMERS,2020,236:9. |
| APA | Zeng, Hui,Huang, Lulin,Tao, Hong,Zhang, Yiwen,&Ding, Kan.(2020).Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells.CARBOHYDRATE POLYMERS,236,9. |
| MLA | Zeng, Hui,et al."Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells".CARBOHYDRATE POLYMERS 236(2020):9. |
入库方式： OAI收割
来源：上海药物研究所
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