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Systematic investigation on the chemical basis of anti-NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

文献类型：期刊论文

作者Liu, Huan1,2; Sun, Zhaolin1,2; Tian, Xiaoting1,2; Feng, Qin3; Guo, Ziqiong1,2; Chen, Shuoji2; Yin, Hao2; Wang, Yangyang1,2; Xu, Zhou1,2; Xie, Like2
刊名BIOMEDICAL CHROMATOGRAPHY
出版日期2020-02-20
页码15
关键词HPLC-Q-TOF intestinal bacteria liver metabolism Qushi Huayu Fang
ISSN号0269-3879
DOI10.1002/bmc.4805
通讯作者Hu, Pei(hupeishtcm@126.com) ; Huang, Chenggang(cghsimm@126.com)
英文摘要Qushi Huayu Fang (QHF) is a clinic-empirical prescription for treating non-alcoholic fatty liver disease (NAFLD) in China, which is composed of five herbs. However, the bioactive constituents responsible for the efficacy of QHF remain unclear. Thus, a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was established and adopted to identify the constituents of QHF, and profile its metabolism in vivo and in vitro. Among the 66 constituents in QHF, only 14 compounds of six structural types were absorbed, and 34 metabolites were generated through eight metabolic pathways. A total of 20 metabolites were first reported, including four organic acids, one iridoid, two flavones, five naphthols, three anthraquinones, and five stilbenes. Glucuronidation and sulfation were the main metabolic pathways, and the intestinal metabolism played an important role in the biotransformation of QHF. Many compounds, especially those detected in the liver, the target organ of QHF, were reported to display the anti-NAFLD activity. This is the first study to explore the constituents of QHF and its metabolism in vivo and in vitro, thus realizing the first step to clarify the chemical basis of QHF qualitatively, and laying the foundation for further research on the anti-NAFLD mechanism of QHF.
WOS关键词FATTY LIVER-DISEASE ; RATS ; MODEL ; ACID ; CONSTITUENTS ; EXPRESSION ; PATHWAY ; PLASMA ; EMODIN ; MS/MS
资助项目Youth Innovation Promotion Association of Chinese Academy of Sciences[2019280] ; Shanghai Science and Technology Development Funds[1840193310] ; Shanghai Science and Technology Development Funds[18401931300] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2019ZX09201001-001-002] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2019ZX09201001-001-008] ; National Natural Science Foundation of China[81973456]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000514490500001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/281231]  
专题中国科学院上海药物研究所
通讯作者Hu, Pei; Huang, Chenggang
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Hai Ke Rd, Shanghai 201203, Peoples R China
3.Shanghai Univ Tradit Chinese Med, Inst Liver Dis, Shuguang Hosp, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Huan,Sun, Zhaolin,Tian, Xiaoting,et al. Systematic investigation on the chemical basis of anti-NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2020:15.
APA Liu, Huan.,Sun, Zhaolin.,Tian, Xiaoting.,Feng, Qin.,Guo, Ziqiong.,...&Huang, Chenggang.(2020).Systematic investigation on the chemical basis of anti-NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,15.
MLA Liu, Huan,et al."Systematic investigation on the chemical basis of anti-NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry".BIOMEDICAL CHROMATOGRAPHY (2020):15.

入库方式： OAI收割

来源：上海药物研究所

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