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Quantitative Proteomics Reveals the Protective Effects of Huangqi Decoction Against Acute Cholestatic Liver Injury by Inhibiting the NF-kappa B/IL-6/STAT3 Signaling Pathway

文献类型：期刊论文

作者Wu, Jia-Sheng1; Liu, Qian4,5,7; Fang, Shan-Hua4,5; Liu, Xing4,5; Zheng, Min1; Wang, Tian-Ming1; Zhang, Hua6; Liu, Ping2,6; Zhou, Hu2,4,5,7; Ma, Yue-Ming1,3
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2020-02-01
卷号19期号:2页码:677-687
关键词intrahepatic cholestasis Huangqi decoction proteomics NF-kappa B IL-6 STAT3
ISSN号1535-3893
DOI10.1021/acs.jproteome.9b00563
通讯作者Zhou, Hu(zhouhu@simm.ac.cn) ; Ma, Yue-Ming(mayueming@shutcm.edu.cn)
英文摘要Intrahepatic cholestasis (IC) is a common syndrome that affects the liver, with treatment options being limited. Huangqi decoction (HQD), a classic herbal medicine, has shown protective effects against IC. In this study, isobaric tags for relative and absolute quantification-based quantitative proteomics was performed to investigate the potential mechanism of action of HQD on alpha-naphthylisothiocyanate (ANIT)-induced IC, resulting in 2796 quantified proteins across all samples, including 270 differentially expressed proteins under HQD treatment. Fuzzy c-means clustering analysis of these 270 proteins assigned the proinflammatory proteins, such as LCN2, SAA1, FGG, FGA, and FGB, to Cluster 1 (upregulated by ANIT, and downregulated by HQD). Functional bioinformatics and protein-protein interaction network analyses indicated that these proinflammatory proteins were involved in the STAT3 signaling pathway. Further real-time PCR and Western blot experiments confirmed that the expression of these proteins was consistent with the proteomic results. Moreover, HQD treatment decreased the phosphorylation of STAT3, induced by ANIT. Western blot experiments revealed that HQD treatment decreased phosphorylation of NF-kappa B and downregulated the expression of the inflammatory gene IL-6 and therefore inhibited the IL-6/STAT3 signaling pathway. In summary, the present study suggested that HQD may ameliorate acute cholestatic liver injury via inhibition of the NF-kappa B/IL-6/STAT3 signaling pathway.
WOS关键词INFLAMMATION ; EXPRESSION ; ALPHA ; ITRAQ ; ACID
资助项目National Natural Science Foundation of China[81503343] ; National Natural Science Foundation of China[81273728] ; Xinglin Young Talent Program[A1-U1820501040218] ; National Key Research and Development Program from the Ministry of Science and Technology of China[2017YFC1700200]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000513085100011
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/281395]  
专题中国科学院上海药物研究所
通讯作者Zhou, Hu; Ma, Yue-Ming
作者单位1.Shanghai Univ Tradit Chinese Med, Dept Pharmacol, Sch Pharm, 1200 Cailun Rd, Shanghai 201203, Peoples R China
2.Shanghai Univ Tradit Chinese Med, E Inst, Shanghai Municipal Educ Comm, 1200 Cailun Rd, Shanghai 201203, Peoples R China
3.Shanghai Univ Tradit Chinese Med, Shanghai Key Lab Compound Chinese Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Dept Analyt Chem, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
5.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
6.Shuguang Hosp, Inst Liver Dis, Minist Educ, Key Lab Liver & Kidney Dis, Shanghai 201203, Peoples R China
7.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Jia-Sheng,Liu, Qian,Fang, Shan-Hua,et al. Quantitative Proteomics Reveals the Protective Effects of Huangqi Decoction Against Acute Cholestatic Liver Injury by Inhibiting the NF-kappa B/IL-6/STAT3 Signaling Pathway[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(2):677-687.
APA Wu, Jia-Sheng.,Liu, Qian.,Fang, Shan-Hua.,Liu, Xing.,Zheng, Min.,...&Ma, Yue-Ming.(2020).Quantitative Proteomics Reveals the Protective Effects of Huangqi Decoction Against Acute Cholestatic Liver Injury by Inhibiting the NF-kappa B/IL-6/STAT3 Signaling Pathway.JOURNAL OF PROTEOME RESEARCH,19(2),677-687.
MLA Wu, Jia-Sheng,et al."Quantitative Proteomics Reveals the Protective Effects of Huangqi Decoction Against Acute Cholestatic Liver Injury by Inhibiting the NF-kappa B/IL-6/STAT3 Signaling Pathway".JOURNAL OF PROTEOME RESEARCH 19.2(2020):677-687.

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来源：上海药物研究所

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