Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies
文献类型：期刊论文
| 作者 | Chen, Zhendong1,2; Gao, Yuxiong1,2; Zhong, Dafang1,2
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2020-03-01 |
| 卷号 | 34期号:3页码:13 |
| 关键词 | chromatographic methods clean-up efficiency derivatization high-resolution chromatography recovery sensitivity |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.4798 |
| 通讯作者 | Zhong, Dafang(dfzhong@simm.ac.cn) |
| 英文摘要 | Chromatographic method has long been recognized as the most widely used separation method in bioanalytical research. However, the relatively low sensitivity of existing chromatographic methods remains a significant challenge, as the requirements for experimental procedures become more demanding. This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean-up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of analytes and enhancing MS ionization efficiency; (iii) optimal LC-MS conditions and appropriate separation mechanism; and (iv) applications of other chromatographic methods, including miniaturized LC, 2D-LC, 2D-GC, and supercritical fluid chromatography. Altogether, this review is devoted to summarizing the recent technologies reported in the literature and providing new strategies for the detection of bioanalytes. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; LC-MS/MS METHOD ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; CLOUD-POINT EXTRACTION ; ELECTROSPRAY-IONIZATION ; HUMAN PLASMA ; HIGH-THROUGHPUT ; RAT PLASMA ; ENANTIOSELECTIVE DETERMINATION |
| 资助项目 | National Natural Science Foundation of China[81521005] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050306] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000511849800001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281675]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhendong,Gao, Yuxiong,Zhong, Dafang. Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies[J]. BIOMEDICAL CHROMATOGRAPHY,2020,34(3):13. |
| APA | Chen, Zhendong,Gao, Yuxiong,&Zhong, Dafang.(2020).Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies.BIOMEDICAL CHROMATOGRAPHY,34(3),13. |
| MLA | Chen, Zhendong,et al."Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies".BIOMEDICAL CHROMATOGRAPHY 34.3(2020):13. |
入库方式： OAI收割
来源：上海药物研究所
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