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Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes

文献类型：期刊论文

作者Han, Hai-Hao2,4; Sedgwick, Adam C.1,3; Shang, Ying2; Li, Na5; Liu, Tingting4; Li, Bo-Han4; Yu, Kunqian4; Zang, Yi4; Brewster, James T., II1; Odyniec, Maria L.3
刊名CHEMICAL SCIENCE
出版日期2020-01-28
卷号11期号:4页码:1107-1113
ISSN号2041-6520
DOI10.1039/c9sc03961a
通讯作者Li, Jia(jli@ecust.edu.cn) ; Sessler, Jonathan L.(sessler@cm.utexas.edu) ; James, Tony D.(chstdj@bath.ac.uk) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要Herein, we report a protein-based hybridization strategy that exploits the host-guest chemistry of HSA (human serum albumin) to solubilize the otherwise cell impermeable ONOO- fluorescent probe Pinkment-OAc. Formation of a HSA/Pinkment-OAc supramolecular hybrid was confirmed by SAXS and solution-state analyses. This HSA/Pinkment-OAc hybrid provided an enhanced fluorescence response towards ONOO(-)versusPinkment-OAc alone, as determined by in vitro experiments. The HSA/Pinkment-OAc hybrid was also evaluated in RAW 264.7 macrophages and HeLa cancer cell lines, which displayed an enhanced cell permeability enabling the detection of SIN-1 and LPS generated ONOO- and the in vivo imaging of acute inflammation in LPS-treated mice. A remarkable 5.6 fold (RAW 264.7), 8.7-fold (HeLa) and 2.7-fold increased response was seen relative to Pinkment-OAc alone at the cellular level and in vivo, respectively. We anticipate that HSA/fluorescent probe hybrids will soon become ubiquitous and routinely applied to overcome solubility issues associated with hydrophobic fluorescent imaging agents designed to detect disease related biomarkers.
WOS关键词ALUMINUM IONS SYNTHESIS ; HOST-GUEST SYSTEM ; X-RAY-SCATTERING ; NITRIC-OXIDE ; OXIDATIVE STRESS ; FREE-RADICALS ; PEROXYNITRITE ; FLUORESCENT ; ANTIOXIDANTS ; ALBUMIN
资助项目National Natural Science Foundation of China[21788102] ; National Natural Science Foundation of China[91853201] ; National Natural Science Foundation of China[21722801] ; National Natural Science Foundation of China[81673489] ; National Natural Science Foundation of China[31871414] ; National Natural Science Foundation of China[U1832144] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; International Cooperation Program of Shanghai Science and Technology Committee[17520750100] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2017319] ; Shanghai Science and Technology Committee[19410712600] ; Fundamental Research Funds for the Central Universities[222201717003] ; Royal Society ; National Institutes of Health[RO1 GM103790] ; Robert A. Welch Foundation[F-0018] ; EPSRC ; University of Bath ; EPSRC[EP/L016354/1]
WOS研究方向Chemistry
语种英语
出版者ROYAL SOC CHEMISTRY
WOS记录号WOS:000510929600019
源URL[http://119.78.100.183/handle/2S10ELR8/281716]  
专题新药研究国家重点实验室
通讯作者Li, Jia; Sessler, Jonathan L.; James, Tony D.; He, Xiao-Peng
作者单位1.Univ Texas Austin, Dept Chem, 105 E 24th St A5300, Austin, TX 78712 USA
2.East China Univ Sci & Technol, Key Lab Adv Mat, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr,Sch C, 130 Meilong Rd, Shanghai 200237, Peoples R China
3.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China
5.Zhanjiang Lab, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China
6.Shanghai Univ, Dept Chem, Ctr Supramol Chem & Catalysis, 99 Shang Da Rd, Shanghai 200444, Peoples R China
推荐引用方式
GB/T 7714		 Han, Hai-Hao,Sedgwick, Adam C.,Shang, Ying,et al. Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes[J]. CHEMICAL SCIENCE,2020,11(4):1107-1113.
APA Han, Hai-Hao.,Sedgwick, Adam C..,Shang, Ying.,Li, Na.,Liu, Tingting.,...&Tian, He.(2020).Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes.CHEMICAL SCIENCE,11(4),1107-1113.
MLA Han, Hai-Hao,et al."Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes".CHEMICAL SCIENCE 11.4(2020):1107-1113.

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来源：上海药物研究所

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