Simultaneous determination of cinobufagin and its five metabolites in rat plasma by LC-MS/MS for characterization of metabolic profiles and pharmacokinetic study
文献类型：期刊论文
| 作者 | Wei, Wenlong; Li, Haojv; Li, Zhenwei; Qu, Hua; Da, Juan; Jin, Xu; Wang, Yingying; Wu, Wanying ; Guo, De-an
|
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2019-11-14 |
| 卷号 | 11期号:42页码:5464-5471 |
| ISSN号 | 1759-9660 |
| DOI | 10.1039/c9ay00807a |
| 通讯作者 | Wu, Wanying(wanyingwu@simm.ac.cm) ; Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | Cinobufagin is one of the main ingredients of Venenum Bufonis and has various pharmacological activities and toxicities. It is very important to simultaneously determine cinobufagin and its metabolites because of its strong physiological activity but it is also very challenging due to its low concentration in blood. In this study, a convenient and efficient method was developed to simultaneously determine cinobufagin and its five metabolites in rat plasma (semi-quantification). BF211, the derivative of bufalin, was added to the plasma sample as an internal standard (IS). An ACQUITY BEH C18 column (2.1 mm x 50 mm, 1.7 mu m) was used for the separation at 40 degrees C, and the mobile phase containing the acetonitrile and water added 0.05% formic acid was run under gradient elution at 0.4 mL min(-1). A LC-MS/MS with an electrospray ionization source in positive ion mode was used for the quantification of cinobufagin and its five metabolites in the mode of multiple reaction monitoring (MRM). The monitored ion pairs were m/z 443.5 -> 365.3 for the transition of cinobufagin, m/z 513.7 -> 145.3 for IS, and m/z 443.2 -> 365.2, 401.2 -> 265.2, and 417.2 -> 363.2 for 3-epi-cinobufagin, desacetylcinobufagin and hydroxyl-desacetylcinobufagin respectively. The calibration curve of cinobufagin showed good linearity in the range of 1.0-200 ng mL(-1) (y = 0.201x + 0.105 and R-2 > 0.990) and the limit of quantitation (LOQ) was 1.0 ng mL(-1). The specificity, accuracy, precision, extraction recovery, matrix effect and stability of the method were satisfactory. According to the results of pharmacokinetic study, cinobufagin was absorbed quickly (T-max = 0.083 +/- 0 h) and the main metabolite was desacetylcinobufagin (C-max = 897.95 +/- 237.35 ng mL(-1)). This pharmacokinetic study could be used for obtaining essential data to interpret the pharmacokinetic-pharmacodynamic relationships of cinobufagin. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINE ; SHEXIANG BAOXIN PILL ; LIU-SHEN-WAN ; CHAN-SU ; BUFADIENOLIDES ; IDENTIFICATION ; PURIFICATION ; KYUSHIN ; PLANT |
| 资助项目 | National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH-K-LN-01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800] |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Spectroscopy |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000493548400012 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281780]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wu, Wanying; Guo, De-an |
| 作者单位 | Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wenlong,Li, Haojv,Li, Zhenwei,et al. Simultaneous determination of cinobufagin and its five metabolites in rat plasma by LC-MS/MS for characterization of metabolic profiles and pharmacokinetic study[J]. ANALYTICAL METHODS,2019,11(42):5464-5471. |
| APA | Wei, Wenlong.,Li, Haojv.,Li, Zhenwei.,Qu, Hua.,Da, Juan.,...&Guo, De-an.(2019).Simultaneous determination of cinobufagin and its five metabolites in rat plasma by LC-MS/MS for characterization of metabolic profiles and pharmacokinetic study.ANALYTICAL METHODS,11(42),5464-5471. |
| MLA | Wei, Wenlong,et al."Simultaneous determination of cinobufagin and its five metabolites in rat plasma by LC-MS/MS for characterization of metabolic profiles and pharmacokinetic study".ANALYTICAL METHODS 11.42(2019):5464-5471. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。