Danhong injection mobilizes endothelial progenitor cells to repair vascular endothelium injury via upregulating the expression of Akt, eNOS and MMP-9
文献类型：期刊论文
| 作者 | Hu, Zhen2,3,4,9; Wang, Hong2,3,4; Fan, Guanwei2,3,4,9; Zhang, Han2,3,4; Wang, Xiaoying2,3,4; Mao, Jingyuan9; Zhao, Yingqiang7; An, Yi1; Huang, Yuhong7; Li, Chuan8 |
| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 61页码:10 |
| 关键词 | Danhong injection Endothelial progenitor cells Mobilization Vascular repair Expression of Akt, eNOS, and MMP-9 |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2019.152850 |
| 通讯作者 | Fan, Guanwei(fgw1005@hotmail.com) ; Gao, Xiumei(gaoxiumei@tjutcm.edu.cn) |
| 英文摘要 | Backgroud: Endothelial progenitor cells (EPCs) have been characterized as one of the key effectors of endothelial healing. The effect of Danhong injection (DHI), the most widely prescribed Chinese medicine for coronary heart disease (CHD), on EPCs mobilization remains unclear. Purpose: We aimed to assess the effect of DHI on EPCs mobilization to repair percutaneous coronary intervention (PCI) induced vascular injury, and to investigate the characteristics and potential mechanism of DHI on EPCs mobilization. Method: Forty-two patients with CHD underwent PCI and received stent implantation were enrolled in a Phase II clinical trials. All patients received routine western medical treatment after PCI, patients of DHI group received DHI in addition. The levels of CECs, cytokines (vWF, IL-6, CRP) and EPCs were analyzed at baseline, post-PCI and after treatment. To investigate the characteristics of DHI on EPCs mobilization, 12 healthy volunteers received intravenous infusion of DHI once and the other 12 received for 7 days. EPCs enumeration were done at a series of time points. At last we tested the effect of DHI and three chemical constituents of DHI (danshensu; lithospermic acid, LA; salvianolic acid D, SaD) on EPCs level and expression of Akt, eNOS and MMP-9 in bone marrow cells of myocardial infarction (MI) mice. Results: In the DHI group the angina symptoms were improved, the levels of cytokines and CECs were reduced; while EPCs population was increased after treatment. In the phase I clinical trials, EPCs counts reached a plateau phase in 9 h and maintained for more than 10 h after a single dose. After continuous administration, EPCs levels plateaued on the 3rd or 4th day, and maintain till 1 day after the withdrawal, then its levels gradually declined. DHI treatment induced a timely dependent mobilization of EPCs. DHI promoted EPCs mobilization via upregulating the expression of Akt, eNOS and MMP-9 in BM. LA and SaD have played a valuable role in EPCs mobilization. Conclusion: These initial results demonstrated that DHI is effective in alleviating endothelial injury and promoting endothelial repair through enhancing EPCs mobilization and revealed the effect feature and possible mechanisms of DHI in mobilizing EPCs. |
| WOS关键词 | PERCUTANEOUS CORONARY INTERVENTION ; ASSOCIATION TASK-FORCE ; 2007 FOCUSED UPDATE ; MYOCARDIAL-INFARCTION ; AMERICAN-COLLEGE ; GUIDELINE UPDATE ; MANAGEMENT ; INDEXES ; ANGINA ; RISK |
| 资助项目 | China National Key Basic Research Program[2012CB518404] ; National Natural Science Funds[81603405] ; National Natural Science Funds[81125024] ; National Natural Science Funds[81630106] ; Ministry of Education development plan of innovation team-study of TCM in prevention and treatment of cardiovascular disease[IRT1276] ; Tianjin science and technology plan project-Tianjin traditional Chinese medicine internal medicine clinical medical research center[15ZXLCSY00020] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000492729600015 |
| 出版者 | ELSEVIER GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/281977]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Fan, Guanwei; Gao, Xiumei |
| 作者单位 | 1.Qingdao Univ, Affiliated Cardiovasc Hosp, 5 Zhiquan Rd, Qingdao 266071, Shandong, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Ticmjin State Key Lab Modern Chinese Med, 312 Anshan West Rd, Tianjin 300193, Peoples R China 3.Tianjin Univ Tradit Chinese Med, Key Res Dept Prescript Component Compatibil, 312 Anshan West Rd, Tianjin 300193, Peoples R China 4.Tianjin Univ Tradit Chinese Med, Key Lab Pharmacol Tradit Chinese Med Formulae, Minist Educ, 312 Anshan West Rd, Tianjin 300193, Peoples R China 5.Univ Alabama Birmingham, Sch Med, Dept Biomed Engn, Birmingham, AL USA 6.Univ Alabama Birmingham, Sch Engn, Birmingham, AL USA 7.Tianjin Univ Tradit Chinese Med, Teaching Hosp 2, 816 Zhenli Rd, Tianjin 300150, Peoples R China 8.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China 9.Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, 314 Anshan West Rd, Tianjin 300193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Zhen,Wang, Hong,Fan, Guanwei,et al. Danhong injection mobilizes endothelial progenitor cells to repair vascular endothelium injury via upregulating the expression of Akt, eNOS and MMP-9[J]. PHYTOMEDICINE,2019,61:10. |
| APA | Hu, Zhen.,Wang, Hong.,Fan, Guanwei.,Zhang, Han.,Wang, Xiaoying.,...&Zhang, Boli.(2019).Danhong injection mobilizes endothelial progenitor cells to repair vascular endothelium injury via upregulating the expression of Akt, eNOS and MMP-9.PHYTOMEDICINE,61,10. |
| MLA | Hu, Zhen,et al."Danhong injection mobilizes endothelial progenitor cells to repair vascular endothelium injury via upregulating the expression of Akt, eNOS and MMP-9".PHYTOMEDICINE 61(2019):10. |
入库方式： OAI收割
来源：上海药物研究所
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