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The characterization of column heating effect in nanoflow liquid chromatography mass spectrometry (nanoLC-MS)-based proteomics

文献类型：期刊论文

作者Li, Bolin1,2; Guo, Fang3; Hu, Hao1; Liu, Ping1; Tan, Minjia1; Pan, Jianyi2; Zhai, Linhui1
刊名JOURNAL OF MASS SPECTROMETRY
出版日期2020
卷号55期号:1页码:10
ISSN号1076-5174
关键词column heating hydrophilic hydrophobic nanoflow liquid chromatography mass spectrometry proteomics
DOI10.1002/jms.4441
通讯作者Pan, Jianyi(jianyi.pan@zstu.edu.cn) ; Zhai, Linhui(zhailinhui@simm.ac.cn)
英文摘要Column heating strategy is often applied in nano-high-performance liquid chromatography-mass spectrometer (nanoHPLC-MS) platform for enhancing the analytical efficiency of peptides or proteins. Nonetheless, the influence effects of column heating in peptides or proteins identification still lack of deep understanding. In this study, a systematic comparison of room temperature (RT) and column heating of nanoHPLC was done. Based on the data, under column heating condition, the backpressure of nanoHPLC can be decreased. Due to the increase of resolution, the peak widths of precursor ion were narrowed. As a result, in MS/MS data acquisition part, more time was spared for MS1 detecting and MS2 fragmenting, which eventually resulted in increased identification of peptides and proteins. Moreover, we also proposed the application scope of column heating by evaluating its influence on sample detection. On one hand, column heating significantly increased the identification of membrane proteins due to more efficient elution of highly hydrophobic peptides compared with RT. On the other hand, heating was not suitable for analyzing short or/and hydrophilic peptides with low retention time, which would be eluted out during sample loading process under high temperature and missed by mass spectrometric detection. In conclusion, our study provides a reference for rational application of column heating in proteomics research.
WOS关键词PRECOLUMN DISPERSION ; TEMPERATURE ; PEPTIDES ; PRESSURE ; PHASE ; PHOSPHORYLATION ; EFFICIENCY ; STRATEGY ; VOLUME ; YEAST
资助项目Innovation Project of Instrument and Equipment Function Development of the Chinese Academy of Sciences[YZ201647] ; Natural Science Foundation of China[31700724] ; Special Project on Precision Medicine under the National Key RD Program[2017YFC0906600] ; Youth Science and Technology Talents in Shanghai Sail Plan of China[16YF1414000] ; Youth Science and Technology Talents in Shanghai Sail Plan of China[17YF1423200]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
出版者WILEY
WOS记录号WOS:000502644500001
源URL[http://119.78.100.183/handle/2S10ELR8/282157]  
专题新药研究国家重点实验室
通讯作者Pan, Jianyi; Zhai, Linhui
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
2.Zhejiang Sci Tech Univ, Sch Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
3.Shanghai Easymass Co Ltd, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Li, Bolin,Guo, Fang,Hu, Hao,et al. The characterization of column heating effect in nanoflow liquid chromatography mass spectrometry (nanoLC-MS)-based proteomics[J]. JOURNAL OF MASS SPECTROMETRY,2020,55(1):10.
APA Li, Bolin.,Guo, Fang.,Hu, Hao.,Liu, Ping.,Tan, Minjia.,...&Zhai, Linhui.(2020).The characterization of column heating effect in nanoflow liquid chromatography mass spectrometry (nanoLC-MS)-based proteomics.JOURNAL OF MASS SPECTROMETRY,55(1),10.
MLA Li, Bolin,et al."The characterization of column heating effect in nanoflow liquid chromatography mass spectrometry (nanoLC-MS)-based proteomics".JOURNAL OF MASS SPECTROMETRY 55.1(2020):10.

入库方式： OAI收割

来源：上海药物研究所

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