中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
In-depth profiling, characterization, and comparison of the ginsenosides among three different parts (the root, stem leaf, and flower bud) of Panax quinquefolius L. by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry

文献类型：期刊论文

作者Wang, Hongda1,3; Zhang, Chunxia1,3; Zuo, Tiantian1,3; Li, Weiwei1,3; Jia, Li1,3; Wang, Xiaoyan1,3; Qian, Yuexin1,3; Guo, Dean2,3; Yang, Wenzhi1,3
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2019-11-01
卷号411期号:29页码:7817-7829
关键词Panax quinquefolius Ginsenoside Q-Orbitrap Mass defect filter Metabolomics
ISSN号1618-2642
DOI10.1007/s00216-019-02180-8
通讯作者Guo, Dean(daguo@simm.ac.cn) ; Yang, Wenzhi(wzyang0504@tjutcm.edu.cn)
英文摘要Despite Panax quinquefolius L. serving as a crucial source for food additives, healthcare products, and herbal medicines, unawareness of the metabolome differences among three parts (root, PQR; stem leaf, PQL; flower bud, PQF) seriously restricts its quality control. Ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry (UHPLC/Q-Orbitrap-MS) was fully utilized to comprehensively identify and compare the ginsenoside compositions among PQR, PQL, and PQF. Metabolite profiling and characterization were performed by coupling reversed-phase UHPLC (a CSH C18 column) and improved untargeted data-dependent MS2 acquisition in the negative mode. A novel vehicle, "Ginsenoside Sieve," was proposed by developing fixed tolerance (10 mDa), discrete mass defect filtering (MDF) based on the m/z features of 499 known ginsenosides, which assisted in the screening of 71 (from 3453 ions), 89 (from 6842), and 84 (from 7369) target precursor masses for PQR, PQL, and PQF, respectively. The newly established data-dependent acquisition (DDA) approach exhibited 14% improvement in characterization of targeted components (using a PQL sample), and comparable performance in identifying the unknown, compared with conventional DDA. We could characterize 347 saponins (147 from PQR, 173 from PQL, and 195 from PQF), and 157 thereof not ever-isolated from the Panax genus. These potentially new saponins have 63 unknown masses. Subsequent untargeted metabolomics analysis unveiled 20 marker saponins, among which m-Rb1, Rb1, Ro, m-Rb2, and m-Rb1 isomer are the most important diagnostic for differentiating the three parts. Conclusively, the established improved DDA represents a potent ginsenoside characterization strategy, and the results obtained in this work would benefit better quality control of P. quinquefolius.
WOS关键词AMERICAN GINSENG ; METABOLOMIC APPROACH ; DISCRIMINATION ; IDENTIFICATION ; STRATEGY ; EXTRACTION ; MARKERS
资助项目National Natural Science Foundation of China[81872996] ; State Key Research and Development Project-Special Research of TCM Modernization[2017YFC1702104] ; State Key Research and Development Project-Special Research of TCM Modernization[2018YFC1707904] ; State Key Research and Development Project-Special Research of TCM Modernization[2018YFC1707905] ; Tianjin Municipal Education Commission Research Project[2017ZD07]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000502969200021
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/282160]  
专题中国科学院上海药物研究所
通讯作者Guo, Dean; Yang, Wenzhi
作者单位1.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, 312 Anshanxi Rd, Tianjin 300193, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China
3.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, Tianjin State Key Lab Modern Chinese Med, 312 Anshanxi Rd, Tianjin 300193, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Hongda,Zhang, Chunxia,Zuo, Tiantian,et al. In-depth profiling, characterization, and comparison of the ginsenosides among three different parts (the root, stem leaf, and flower bud) of Panax quinquefolius L. by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(29):7817-7829.
APA Wang, Hongda.,Zhang, Chunxia.,Zuo, Tiantian.,Li, Weiwei.,Jia, Li.,...&Yang, Wenzhi.(2019).In-depth profiling, characterization, and comparison of the ginsenosides among three different parts (the root, stem leaf, and flower bud) of Panax quinquefolius L. by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(29),7817-7829.
MLA Wang, Hongda,et al."In-depth profiling, characterization, and comparison of the ginsenosides among three different parts (the root, stem leaf, and flower bud) of Panax quinquefolius L. by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.29(2019):7817-7829.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。