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Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity

文献类型：期刊论文

作者Ding, Chunyong2; Wang, Fengyang1; Dang, Yijing1; Xu, Zhiai1; Li, Lingling1; Lai, Yi1; Yu, Haijun3; Luo, Yi4; Huang, Ruimin3; Zhang, Ao2
刊名ANALYTICAL CHEMISTRY
出版日期2019-12-17
卷号91期号:24页码:15577-15584
ISSN号0003-2700
DOI10.1021/acs.analchem.9b03600
通讯作者Ding, Chunyong(chding@simm.ac.cn) ; Xu, Zhiai(zaxu@chem.ecnu.edu.cn) ; Zhang, Wen(wzhang@chem.ecnu.edu.cn)
英文摘要The accurate detection of tumorous methylglyoxal (MGO) and its detoxifier glyoxalase 1 (GLO1) in living systems is critical for understanding their roles in tumor initiation and progression. To date, the in situ fluorescence detection of endogenous MGO and GLO1 in tumor has not been reported. Herein we developed a near-infrared (NIR) fluorescent probe MEBTD to specifically detect tumorous MGO. Compared with previously reported MGO fluorescent probes, MEBTD exhibits several distinct advantages, including NIR emission, high selectivity with an MGO detection limit of 18 nM, and a 131-fold off-on ratio. The probe could sense GLO1 activity and monitor the therapeutic effect of GLO1 inhibitors by imaging tumorous MGO in a both a real-time and in situ manner, demonstrating that the biological effect of GLO1 inhibitors is dependent on the GLO1 activity. Furthermore, MEBTD enables the visualization of tumorous MGO induced by GLO1 inhibitors in vivo. To the best of our knowledge, MEBTD is the first NIR fluorescent probe for specifically imaging tumorous MGO in living animals, indicating the promising potential for tumor diagnosis and therapeutic evaluation.
WOS关键词ALZHEIMERS-DISEASE ; I INHIBITION ; APOPTOSIS ; PLASMA ; 3-DEOXYGLUCOSONE ; MECHANISM ; GLYCATION ; OBESITY ; STRESS ; CELLS
资助项目National Natural Science Foundation of China[21675055] ; National Natural Science Foundation of China[81402790] ; National Natural Science Foundation of China[21775046] ; National Natural Science Foundation of China[21635003] ; Personalized Medicines Molecular Signature-based Drug Discovery and Development of the Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020320] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program, China[2018ZX09711002] ; State Key Laboratory of Drug Research[SIMM1903KF-13] ; One Hundred Talent Program of the Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000503910600034
源URL[http://119.78.100.183/handle/2S10ELR8/282295]  
专题新药研究国家重点实验室
通讯作者Ding, Chunyong; Xu, Zhiai; Zhang, Wen
作者单位1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Dalian Univ Technol, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Chunyong,Wang, Fengyang,Dang, Yijing,et al. Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity[J]. ANALYTICAL CHEMISTRY,2019,91(24):15577-15584.
APA Ding, Chunyong.,Wang, Fengyang.,Dang, Yijing.,Xu, Zhiai.,Li, Lingling.,...&Zhang, Wen.(2019).Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity.ANALYTICAL CHEMISTRY,91(24),15577-15584.
MLA Ding, Chunyong,et al."Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity".ANALYTICAL CHEMISTRY 91.24(2019):15577-15584.

入库方式： OAI收割

来源：上海药物研究所

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