Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)
文献类型：期刊论文
| 作者 | Gao, Yan2,3; Lee, Hyojin4; Kwon, Oh Kwang2,3; Tan, Minjia5 ; Kim, Ki-Tae1,4; Lee, Sangkyu2,3
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2019-10-01 |
| 卷号 | 18期号:10页码:3762-3769 |
| 关键词 | lysine succinylation Danio rerio post-translational modification global proteomics |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.9b00462 |
| 通讯作者 | Kim, Ki-Tae(ktkim@seoultech.ac.kr) ; Lee, Sangkyu(sangkyu@knu.ac.kr) |
| 英文摘要 | Lysine succinylation (Ksu) is a novel identified post-translational modification that is conserved from prokaryotes to eukaryotes. As a kind of acylation, Ksu was reported to have different functions than other acylations at lysine residues. However, recent studies on Ksu have mainly focused on plants and bacteria. Ksu studies in vertebrates are still rare; thus, the biological function of Ksu in mammals needs to be studied further. In this study, we performed global Ksu mapping in Danio rerio (zebrafish) using mass spectrometry-based proteomics with the enrichment of Ksu peptides by immunoprecipitation technology. As a result, we identified 552 Ksu sites in 164 proteins. The raw data are available via ProteomeXchange with the identifier PXD013173. Compared with our previous studies on lysine acetylation and crotonylation, Ksu plays a major role in diverse metabolic processes such as carbon metabolism and the tricarboxylic acid circle. In addition, we defined five new succinylation motifs: (su)KA, (su)KxxxxA, (su)KxxxxL, (su)KxA, and (su)KxV. In conclusion, our results provide a proteome-wide database to study Ksu in zebrafish, and our bioinformatics results facilitated the understanding of the role of Ksu in central metabolism. |
| WOS关键词 | PYRUVATE-KINASE M2 ; POSTTRANSLATIONAL MODIFICATIONS ; ACETYLATION ; SIRT5 ; DESUCCINYLATION ; IDENTIFICATION ; CROTONYLATION ; MALONYLATION ; PROTEINS ; MYOSIN |
| 资助项目 | Kyungpook National University Bokhyeon Research Fund, 2017 ; Korea Ministry of Environment (MOE) through the Environmental Health Action Program[2016001370001] ; Korea Basic Science Institute (KBSI) National Research Facilities & Equipment Center (NFEC) - Korea government (Ministry of Education)[2019RIA6C1010001] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000489200400020 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/282699]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Kim, Ki-Tae; Lee, Sangkyu |
| 作者单位 | 1.Seoul Natl Univ Sci & Technol, Dept Environm Engn, Seoul 01811, South Korea 2.Kyungpook Natl Univ, BK21 Plus KNU Multiom Based Creat Drug Res Team, Coll Pharm, Daegu 41566, South Korea 3.Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea 4.Seoul Natl Univ Sci & Technol, Dept Energy & Environm Engn, Seoul 01811, South Korea 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yan,Lee, Hyojin,Kwon, Oh Kwang,et al. Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(10):3762-3769. |
| APA | Gao, Yan,Lee, Hyojin,Kwon, Oh Kwang,Tan, Minjia,Kim, Ki-Tae,&Lee, Sangkyu.(2019).Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio).JOURNAL OF PROTEOME RESEARCH,18(10),3762-3769. |
| MLA | Gao, Yan,et al."Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)".JOURNAL OF PROTEOME RESEARCH 18.10(2019):3762-3769. |
入库方式： OAI收割
来源：上海药物研究所
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