comprehensiveprofilingoflysineacetylomeinstaphylococcusaureus
文献类型：期刊论文
| 作者 | Zhang Yi; Wu Zhixiang; Wan Xuelian; Liu Ping; Zhang Jibao; Ye Yang ; Zhao Yingming; Tan Minjia
|
| 刊名 | sciencechinachemistry
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:5页码:732 |
| 关键词 | LARGE GENE LISTS CHROMATIN MODIFICATIONS METABOLIC ENZYMES ESCHERICHIA-COLI ACETYLATION IDENTIFICATION PROTEINS DATABASE REVEALS proteomics post-translational modification acetylation Staphylococcus aureus |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-014-5100-4 |
| 英文摘要 | The Gram-positive bacterium Staphylococcus aureus (S. aureus) is a wide spread common opportunistic pathogen that causes a wide variety of infectious diseases, from benign skin infections to life-threatening diseases such as the methicillin-resistant Staphylococcus aureus (MRSA) infection. Although emerging evidence suggests that lysine acetylation may play critical roles in bacterial physiology, the atlas of acetylome in S. aureus has not been studied. To comprehensively profile protein lysine acetylation in S. aureus, we used an integrated approach that combined immune affinity peptide enrichment using anti-lysine acetylation antibody, high-pH HPLC fractionation, and HPLC/mass spectrometry analysis. This study led to the identification of 1361 non-redundant acetylation sites on 412 proteins found in a search of S. aureus protein database extracted from the Swiss-Prot database. We further performed bioinformatic analysis to characterize this modification, including gene ontology annotation, protein-protein interaction, and domain analysis of the acetylation sites. We found that the acetylated proteins were enriched in multiple biological pathways, such as ribosomal function and energy metabolism. Our data provides a rich source for functional studies of lysine acetylation in S. aureus. |
| 资助项目 | [National Science and Technology Major Project of the Ministry of Science and Technology of China] ; [National Natural Science Foundation of China] ; [Shanghai Pujiang Program] ; [China Postdoctoral Science Foundation] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/282814]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Yi,Wu Zhixiang,Wan Xuelian,et al. comprehensiveprofilingoflysineacetylomeinstaphylococcusaureus[J]. sciencechinachemistry,2014,57(5):732. |
| APA | Zhang Yi.,Wu Zhixiang.,Wan Xuelian.,Liu Ping.,Zhang Jibao.,...&Tan Minjia.(2014).comprehensiveprofilingoflysineacetylomeinstaphylococcusaureus.sciencechinachemistry,57(5),732. |
| MLA | Zhang Yi,et al."comprehensiveprofilingoflysineacetylomeinstaphylococcusaureus".sciencechinachemistry 57.5(2014):732. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。