crystalstructuredeterminationofachimericfabfbyxrd
文献类型：期刊论文
| 作者 | Li Ke1; Li Li2; Xu Yechun2
|
| 刊名 | nuclearscienceandtechniques
 |
| 出版日期 | 2017 |
| 卷号 | 028期号:009页码:123 |
| 关键词 | 晶体结构 嵌合体 XRD 广谱抗菌活性 测定 药物设计 生产菌株 PTM |
| ISSN号 | 1001-8042 |
| 英文摘要 | Beta-ketoacyl-acyl-carrier-protein synthase II, an important enzyme in biosynthesis of bacterial fatty acid, is an attractive target in antibacterial drug design. Platen- simycin （PTM）, produced by Streptomyces platensis, has a strong, broad-spectrum Gram-positive antibacterial activity by selectively targeting to FabF but exhibits no inhibition to the FabF from Streptornyces platensis （spFabF）. To study the self-resistance mechanism within the PTM-pro- ducing strain and provide hint for development of novel antibiotics, it is imperative to solve the structure of spFabF and elucidate the difference between spFabF and other FabFs which are not resistant to PTM. To this end, we constructed four chimeric FabFs based on the sequence of spFabF and its homologous protein after the expression of wide-type spFabF was failed. The crystal structure of one chimera, js200FabF, of 91.2% sequence identity to spFabF, was solved. A structure comparison of js200FabF with a PTM-bound FabF suggested that three loops nearby the catalytic site might play key roles in preventing the binding of PTM to spFabF. The results provide an encouraging basis for further studies on the self-resistance mechanismand structure-based design of novel antibiotics targeting FabFs. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/283349]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.上海大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Li Ke,Li Li,Xu Yechun. crystalstructuredeterminationofachimericfabfbyxrd[J]. nuclearscienceandtechniques,2017,028(009):123. |
| APA | Li Ke,Li Li,&Xu Yechun.(2017).crystalstructuredeterminationofachimericfabfbyxrd.nuclearscienceandtechniques,028(009),123. |
| MLA | Li Ke,et al."crystalstructuredeterminationofachimericfabfbyxrd".nuclearscienceandtechniques 028.009(2017):123. |
入库方式： OAI收割
来源：上海药物研究所
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