Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells
文献类型：期刊论文
| 作者 | Li Dong1; Song Xiaoyi2; Yue Qingxi2; Cui Yajun3; Liu Miao2; Feng Lixing2; Wu Wanying2 ; Jiang Baohong2; Yang Min2; Qu Xiaobo1
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| 刊名 | Chinese Journal of Natural Medicines
 |
| 出版日期 | 2015 |
| 卷号 | 13期号:1页码:41 |
| 关键词 | INDUCED APOPTOSIS INHIBITS ANGIOGENESIS UP-REGULATION CYCLE ARREST CANCER-CELLS PHOSPHORYLATION ACCUMULATION DEGRADATION RESISTANCE MECHANISM Gambogic acid MDA-MB-231 cells Anti-cancer Proteomic Bioinformatic |
| ISSN号 | 2095-6975 |
| 英文摘要 | Gambogic acid (GA) is an anticancer agent in phase IIb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins of GA in cancer cells using proteomic method and establish possible network using bioinformatic analysis. Cytotoxicity and anti-migration effects of GA in MDA-MB-231 cells were checked using MTT assay, flow cytometry, wound migration assay, and chamber migration assay. Possible target-related proteins of GA at early (3 h) and late stage (24 h) of treatment were searched using a proteomic technology, two-dimensional electrophoresis (2-DE). The possible network of GA was established using bioinformatic analysis. The intracellular expression levels of vimentin, keratin 18, and calumenin were determined using Western blotting. GA inhibited cell proliferation and induced cell cycle arrest at G2/M phase and apoptosis in MDA-MB-231 cells. Additionally, GA exhibited anti-migration effects at non-toxic doses. In 2-DE analysis, totally 23 possible GA targeted proteins were found, including those with functions in cytoskeleton and transport, regulation of redox state, metabolism, ubiquitin-proteasome system, transcription and translation, protein transport and modification, and cytokine. Network analysis of these proteins suggested that cytoskeleton-related proteins might play important roles in the effects of GA. Results of Western blotting confirmed the cleavage of vimentin, increase in keratin 18, and decrease in calumenin levels in GA-treated cells. In summary, GA is a multi-target compound and its anti-cancer effects may be based on several target-related proteins such as cytoskeleton-related proteins. |
| 资助项目 | [Twelfth Five-Year National Science & Technology Support Program] ; [Shanghai Science & Technology Support Program] ; [China Postdoctoral Science Foundation Funded Project] ; [Shanghai Postdoctoral Scientific Program] ; [Sanofi-Aventis-Shanghai Institutes for Biological Sciences Scholarship Program, the National Nature Science Foundation] ; [Postdoctor Research Program of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/283658]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.长春中医药大学 2.中国科学院上海药物研究所 3.上海中医药大学 |
| 推荐引用方式 GB/T 7714 | Li Dong,Song Xiaoyi,Yue Qingxi,et al. Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells[J]. Chinese Journal of Natural Medicines,2015,13(1):41. |
| APA | Li Dong.,Song Xiaoyi.,Yue Qingxi.,Cui Yajun.,Liu Miao.,...&Guo Dean.(2015).Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells.Chinese Journal of Natural Medicines,13(1),41. |
| MLA | Li Dong,et al."Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells".Chinese Journal of Natural Medicines 13.1(2015):41. |
入库方式： OAI收割
来源：上海药物研究所
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