Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry for Quantification of Glycyrrhetic Acid in Human Plasma
文献类型：期刊论文
| 作者 | TIAN Li1; GAO Xiaoli2; CHEN Xiaoyan3 ; ZHONG Dafang3; ZHANG Yifan3; DAI Xiaojian3
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| 刊名 | Chemical Research in Chinese Universities
 |
| 出版日期 | 2011 |
| 卷号 | 27期号:2页码:185 |
| 关键词 | 18-BETA-GLYCYRRHETINIC ACID METABOLISM COMPONENT Liquid chromatography-tandem mass spectrometry(LC-MS/MS) Glycyrrhetic acid Pharmacokinetics |
| ISSN号 | 1005-9040 |
| 英文摘要 | A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) for the determination of glycyrrhetic acid in human plasma with ginsenoside Rh(2) as internal standard was developed and validated. The plasma samples were prepared via liquid-liquid extraction with ethyl acetate. Chromatographic separation was accomplished on a Venusil MP-C(18)(50 mmx2.1 mm, 5 mu m i.d.) column at 25 degrees C. The mobile phase consisted of acetonitrile/5 mmol.L(-1) ammonium acetate(10:90, volume ratio) at a flow rate of 0.4 mL/min. Negative electrospray ionization was utilized as the ionization source. Glycyrrhetic acid and internal standard were determined via the mutiple reaction monitoring of precursor -> production ion transitions at m/z 469 -> 425, 409 and m/z 621 -> 161, respectively. Each sample was chromatographed within 2.5 min. The lower limit of quantification was 0.50 ng/mL for 200 mu L of plasma sample and the linear range was from 0.50 ng/mL to 800 ng/mL. The intra- and inter-day precisions were less than 8.76% in terms of relative standard deviation(RSD), and the accuracy was within a range of -3.25%-1.32% in terms of relative error(RE). The method was successfully applied to the pharmacokinetic studies of glycyrrhetic acid in healthy male Chinese volunteers after a single oral administration of 75 mg of glycyrrhizin. |
| 资助项目 | [Xinjiang Vurge Autonomous Region(China)] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/283817]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.College of Trditional Chinese Medicine,Xinjiang Medical University 2.College of Pharmacy,Xinjiang Medical University 3.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | TIAN Li,GAO Xiaoli,CHEN Xiaoyan,et al. Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry for Quantification of Glycyrrhetic Acid in Human Plasma[J]. Chemical Research in Chinese Universities,2011,27(2):185. |
| APA | TIAN Li,GAO Xiaoli,CHEN Xiaoyan,ZHONG Dafang,ZHANG Yifan,&DAI Xiaojian.(2011).Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry for Quantification of Glycyrrhetic Acid in Human Plasma.Chemical Research in Chinese Universities,27(2),185. |
| MLA | TIAN Li,et al."Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry for Quantification of Glycyrrhetic Acid in Human Plasma".Chemical Research in Chinese Universities 27.2(2011):185. |
入库方式： OAI收割
来源：上海药物研究所
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