danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro
文献类型：期刊论文
| 作者 | Zhou Rong1; Wang Ling1; Xu Xing2; Chen Jing2 ; Hu Lihong2; Chen Lili2; Shen Xu1
|
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:8页码:1061 |
| 关键词 | 1 8-dihydroxyanthraquinone (danthron) traditional Chinese medicine AMPK SREBP1c fatty acid synthetase cholesterol triglyceride glucose consumption |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim: To discover the active compound on AMP-activated protein kinase (AMPK) activation and investigate the effects of the active compound 1,8-dihydroxyanthraquinone (danthron) from the traditional Chinese medicine rhubarb on AMPK-mediated lipid and glucose metabolism in vitro. Methods: HepG2 and C2C12 cells were used. Cell viability was determined using MTT assay. Real-time PCR was performed to measure the gene expression. Western blotting assay was applied to investigate the protein phosphorylation level. Enzymatic assay kits were used to detect the total cholesterol (TC), triglyceride (TG) and glucose contents. Results: Danthron (0.1, 1, and 10 μmol/L) dose-dependently promoted the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in both HepG2 and C2C12 cells. Meanwhile, danthron treatment significantly reduced the lipid synthesis related sterol regulatory element-binding protein 1c (SREBP1c) and fatty acid synthetase (FAS) gene expressions, and the TC and TG levels. In addition, danthron treatment efficiently increased glucose consumption. The actions of danthron on lipid and glucose metabolism were abolished or reversed by co-treatment with the AMPK inhibitor compound C. Conclusion: Danthron effectively reduces intracellular lipid contents and enhanced glucose consumption in vitro via activation of AMPK signaling pathway. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/284020]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.华东理工大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Zhou Rong,Wang Ling,Xu Xing,et al. danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro[J]. actapharmacologicasinica,2013,34(8):1061. |
| APA | Zhou Rong.,Wang Ling.,Xu Xing.,Chen Jing.,Hu Lihong.,...&Shen Xu.(2013).danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro.actapharmacologicasinica,34(8),1061. |
| MLA | Zhou Rong,et al."danthronactivatesampactivatedproteinkinaseandregulateslipidandglucosemetabolisminvitro".actapharmacologicasinica 34.8(2013):1061. |
入库方式： OAI收割
来源：上海药物研究所
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