apoptosisinitiatedbycarbontetrachlorideinmitochondriaofratprimaryculturedhepatocytes
文献类型：期刊论文
| 作者 | Cai Yan; Gong Likun ; Qi Xinming; Li Xianghong; Ren Jin
|
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:8页码:969 |
| 关键词 | CYTOCHROME-C OXIDATIVE STRESS ENDONUCLEASE-G CELL-DEATH LIVER TOXICITY PROTEINS RELEASE carbon tetrachloride hepatocytes mitochondria caspase 3 cytochrome c |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2005.00143.x |
| 英文摘要 | Aim: To investigate the mitochondria-initiated apoptosis pathway involved in Carbon tetrachloride (CCl4) hepatotoxicity in vitro. Methods: Several cytotoxicity endpoints, including WST-8 metabolism, lactate dehydrogenase leakage and morphological changes, were examined. The 5,5'-dithio-bis(2-nitrobenzoic acid) reaction was used to measure reduced glutathione level, and the malondialdehyde level was determined using the thiobarbituric acid assay. The release of cytochrome c and Bcl-X-L was detected by Western blot. Caspase-3 activity was measured using the fluorogenic substrate Ac-DEVD-AMC. DNA fragmentation was used to evaluate cell apoptosis. Results: A time- and dose-dependent decrease in cellular glutathione content was observed, along with a concomitant increase in malondialdehyde levels following the application of CCl4. Caspase 3 activity was stimulated at all doses of CCl4, with the most significant activation at 3 mmol/L. Cytochrome c was released obviously after CCl4 treatment. A time-dependent decrease in Bcl-X-L expression was observed. DNA fragmentation results revealed apoptosis and necrosis following CCl4 treatment. Conclusion: Oxidative damage is one of the essential mechanisms of CCl4 hepatotoxicity, which triggers apoptosis via the mitochondria-initiated pathway. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/285281]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Cai Yan,Gong Likun,Qi Xinming,et al. apoptosisinitiatedbycarbontetrachlorideinmitochondriaofratprimaryculturedhepatocytes[J]. actapharmacologicasinica,2005,26(8):969. |
| APA | Cai Yan,Gong Likun,Qi Xinming,Li Xianghong,&Ren Jin.(2005).apoptosisinitiatedbycarbontetrachlorideinmitochondriaofratprimaryculturedhepatocytes.actapharmacologicasinica,26(8),969. |
| MLA | Cai Yan,et al."apoptosisinitiatedbycarbontetrachlorideinmitochondriaofratprimaryculturedhepatocytes".actapharmacologicasinica 26.8(2005):969. |
入库方式： OAI收割
来源：上海药物研究所
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