血管内皮生长因子受体i酪氨酸激酶的克隆表达和功能
文献类型：期刊论文
| 作者 | 庄书斐; 叶其壮 |
| 刊名 | 生物化学与生物物理学报
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:1页码:39 |
| 关键词 | PHOSPHORYLATION SITES VEGF-RECEPTOR SH2 DOMAIN BINDING FLT-1 IDENTIFICATION PROTEINS KDR vascular endothelial growth factor receptor tyrosine kinase fusion protein |
| ISSN号 | 0582-9879 |
| 英文摘要 | Vascular endothelial growth factor receptor 1 (Flt1) plays an important role in angiogenesis. It was hypothesized that, upon binding to VEGF, Flt1 tyrosine kinase underwent dimerization and initiated the signal transduction in VEGF/VEGF receptor system. In this report, a soluble active Flt1 tyrosine kinase domain expressed in E. coli was obtained, and its properties were partly characterized. The eDNA of Flt1 tyrosine kinase domain was obtained from the total RNA extracted from human liver cancer tissues by using RT-PCR, and was cloned to vector pGEX-KG. A soluble active GST-fusion protein of Flt1 tyrosine kinase domain (GST-F) was obtained from E. coli BL21 (DE3) pLysS. Athough it was reported that GST-F contains no phosphorylation site, it did autophospliorylate in vitro. Mg2+ and Mn2+ were essential for the activity. It was also found that GST-F phosphorylated a synthesized substrate PolyE4Y, but nut MBP and Sre-related-peptide. The optimal Mg2+ and Mn2+ concentration for polyE4Y phosphorylation was 15 mmol/L and 0.5 mmol/L, respectively. This work is helpful for developing the new anti-cancer drugs. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/285308]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | 庄书斐,叶其壮. 血管内皮生长因子受体i酪氨酸激酶的克隆表达和功能[J]. 生物化学与生物物理学报,2002,34(1):39. |
| APA | 庄书斐,&叶其壮.(2002).血管内皮生长因子受体i酪氨酸激酶的克隆表达和功能.生物化学与生物物理学报,34(1),39. |
| MLA | 庄书斐,et al."血管内皮生长因子受体i酪氨酸激酶的克隆表达和功能".生物化学与生物物理学报 34.1(2002):39. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。