highthroughputscreeningforsmallmoleculeinhibitorsofthetypeiinterferonsignalingpathway
文献类型：期刊论文
| 作者 | Yuliantie Elita1; Dai Xinchuan1; Yang Dehua1 ; Crack Peter J2; Wang Mingwei1
|
| 刊名 | actapharmaceuticasinicab
 |
| 出版日期 | 2018 |
| 卷号 | 000期号:006页码:889 |
| 关键词 | High-throughput screening Interferon α receptor Secreted embryonic alkaline phosphatase JAK-STAT IFN regulatory factor Inhibitor |
| ISSN号 | 2211-3835 |
| 英文摘要 | Interferons(IFNs) are cytokines with fundamental roles in resistance to infections, cancer and other diseases. Type-I IFNs, interferon α(IFN-α) and interferon β(IFN-β), act through a shared receptor complex(IFNAR) comprised of IFNAR1 and IFNAR2 subunits. Binding of type-I IFN to IFNAR1 will robustly activate Janus activated kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway. Aberrant activation of the type-I IFN response results in a spectrum of disorders called interferonopathies. The purpose of this research is to develop an assay for high-throughput screening(HTS) of small molecule inhibitors of the type-I IFN signaling pathway. Inhibition of type-I IFN signaling can be beneficial in terms of therapeutic use and understanding the underlying mechanism of action. We report here a HTS campaign with the secreted embryonic alkaline phosphatase(SEAP) reporter gene assay against 32,000 compounds which yielded 25 confirmed hits. These compounds were subsequently characterized for their cytotoxicity, effects on STAT phosphorylation and activities in IFN regulatory factor(IRF) transcription. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/285864]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国科学院上海药物研究所 2.墨尔本大学 |
| 推荐引用方式 GB/T 7714 | Yuliantie Elita,Dai Xinchuan,Yang Dehua,et al. highthroughputscreeningforsmallmoleculeinhibitorsofthetypeiinterferonsignalingpathway[J]. actapharmaceuticasinicab,2018,000(006):889. |
| APA | Yuliantie Elita,Dai Xinchuan,Yang Dehua,Crack Peter J,&Wang Mingwei.(2018).highthroughputscreeningforsmallmoleculeinhibitorsofthetypeiinterferonsignalingpathway.actapharmaceuticasinicab,000(006),889. |
| MLA | Yuliantie Elita,et al."highthroughputscreeningforsmallmoleculeinhibitorsofthetypeiinterferonsignalingpathway".actapharmaceuticasinicab 000.006(2018):889. |
入库方式： OAI收割
来源：上海药物研究所
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