lgh00031anovelorthoquinonoidinhibitorofcelldivisioncycle25binhibitshumancancercellsviarosgeneration
文献类型：期刊论文
| 作者 | Yubo ZHOU ; Xu FENG; Lina WANG; Junqing DU; Yueyang ZHOU; Haiping YU; Yi ZANG; Jingya LI; Jia LI
|
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2009 |
| 卷号 | 30期号:9页码:1359 |
| 关键词 | CDC25 PHOSPHATASES TRANSGENIC MICE IN-VIVO DISCOVERY SPECIFICITY MECHANISM ANALOGS |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim: To discover novel cell division cycle 25 (CDC25) B inhibitors and elucidate the mechanisms of inhibition in cancer cells. Methods: Cell growth inhibition was detected by MTT assay, the cell cycle was analyzed by flow cytometry, and protein expression and phosphorylation was examined by Western blot analysis. Results: LGH00031 inhibited CDC25B irreversibly in vitro in a dose-dependent manner, and impaired the proliferation of tumor cell lines. In synchronized HeLa cells, LGH00031 delayed the cell cycle progression at the G 2 in vitro relied on the existence of 1,4-dithiothreitol (DTT) or dihydrolipoic acid and oxygen. The oxygen free radical scavenger catalase and superoxide dismutase reduced the inactivation of CDC25 by LGH00031, confirming that reactive oxygen species (ROS) mediate the inactivation process in vitro . LGH00031 accelerated cellular ROS production in a dose-dependent manner, and N -acetyl cysteine (NAC) markedly decreased the ROS production induced by LGH00031. Correspondingly, the LGH00031-induced decrease in cell viability and cell cycle arrest, cyclin B1 protein level, and phosphorylation of CDK1 tyrosine 15 were also rescued by NAC that decreased ROS production. Conclusion: The activity of LGH00031 at the molecular and cellular level is mediated by ROS. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/285926]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Yubo ZHOU,Xu FENG,Lina WANG,et al. lgh00031anovelorthoquinonoidinhibitorofcelldivisioncycle25binhibitshumancancercellsviarosgeneration[J]. actapharmacologicasinica,2009,30(9):1359. |
| APA | Yubo ZHOU.,Xu FENG.,Lina WANG.,Junqing DU.,Yueyang ZHOU.,...&Jia LI.(2009).lgh00031anovelorthoquinonoidinhibitorofcelldivisioncycle25binhibitshumancancercellsviarosgeneration.actapharmacologicasinica,30(9),1359. |
| MLA | Yubo ZHOU,et al."lgh00031anovelorthoquinonoidinhibitorofcelldivisioncycle25binhibitshumancancercellsviarosgeneration".actapharmacologicasinica 30.9(2009):1359. |
入库方式： OAI收割
来源：上海药物研究所
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