effectsofhuperzineaonlivercytochromep450inrats
文献类型：期刊论文
| 作者 | Ma Xiaochao1; Wang Haixue2; Xin Jian1; Zhang Ting1; Tu Zenghong1 |
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:8页码:831 |
| 关键词 | ALZHEIMERS-DISEASE OXIDATION ENZYMES MICROSOMES CHEMICALS TACRINE huperzine A cytochrome P-450 cytochrome P-450CYP1A2 liver rat |
| ISSN号 | 1671-4083 |
| 英文摘要 | AIM: To predict possible drug interaction and assure safety medication of huperzine A (HupA). METHODS: The effects of HupA on activities and expressions of cytochrome P-450 (CYP) were examined. Liver microsomes and total mRNA were prepared from rats treated orally with 0, 0.1 (pharmacological dose), 1, or 2 mg/kg huperzine A for 2 weeks. Phenobarbital, 3-methylcholanthrene (3-MC), ethanol, and dexamethasone were used as positive controls. Total CYP protein was assayed by carbon monoxide difference spectrum. Activity of isoenzyme was detected with specific probe. Expression of CYP protein and mRNA was analyzed with Western blot and RT-PCR. RESULTS: No changes of isoenzyme expression and catalytic activities were found in rats treated with 0.1 mg/kg huperzine A. Huperzine A 1, 2 mg/kg parallelly increased CYP1A2 activity, protein and mRNA, although they were minor when contrasted with 3-MC. Huperzine A 1, 2 mg/kg got no effects on CYP2C11, CYP2B1/2, 2E1 and 3A. CONCLUSION: Activity and expression of liver CYP isoenzymes were not affected in rats treated with pharmacological dose of HupA, but HupA at toxicological dose may elicit a slight inductive response of CYP1A2. The CYP1A2 induction produced by HupA is related to transcription enhancement. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/286991]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国科学院上海药物研究所 2.复旦大学 |
| 推荐引用方式 GB/T 7714 | Ma Xiaochao,Wang Haixue,Xin Jian,et al. effectsofhuperzineaonlivercytochromep450inrats[J]. actapharmacologicasinica,2003,24(8):831. |
| APA | Ma Xiaochao,Wang Haixue,Xin Jian,Zhang Ting,&Tu Zenghong.(2003).effectsofhuperzineaonlivercytochromep450inrats.actapharmacologicasinica,24(8),831. |
| MLA | Ma Xiaochao,et al."effectsofhuperzineaonlivercytochromep450inrats".actapharmacologicasinica 24.8(2003):831. |
入库方式： OAI收割
来源：上海药物研究所
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