abindingblockionselectivemechanismrevealedbyanakselectivechannel
文献类型：期刊论文
| 作者 | Yu Jie2; Zhang Bing3; Zhang Yixiao2; Xu Congqiao1; Zhuo Wei2; Ge Jingpeng2; Li Jun1; Gao Ning2; Li Yang3 ; Yang Maojun2
|
| 刊名 | proteincell
 |
| 出版日期 | 2018 |
| 卷号 | 009期号:007页码:629 |
| 关键词 | 离子选择 选择机制 约束力 隧道 联合结构 膜蛋白质 MscS 阴离子 |
| ISSN号 | 1674-800X |
| 英文摘要 | Mechanosensitive （MS） channels are extensively stud- ied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the Ynal channel as the Na^＋/K^＋ cation-selec- tive MS channel and solved its structure at 3.8 A by cryo- EM single-particle method. Ynal exhibits low conduc- tance among the family of MS channels in E. coil, and shares a similar overall heptamer structure fold with previously studied MscS channels. By combining structural based mutagenesis, quantum mechanical and electrophysiological characterizations, we revealed that ion selective filter formed by seven hydrophobic methionine （Ynal^Met158） in the transmembrane pore determined ion selectivity, and both ion selectivity and gating of Ynal channel were affected by accompanying anions in solution. Further quantum simulation and functional validation support that the distinct binding energies with various anions to Ynal^Met158 facilitate Na^＋/K^＋ pass through, which was defined as binding-block mechanism. Our structural and functional studies provided a new perspective for understanding the mechanism of how MS channels select ions driven by mechanical force. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/287432]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.清华大学 2.School of Life Sciences,Tsinghua University 3.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Yu Jie,Zhang Bing,Zhang Yixiao,et al. abindingblockionselectivemechanismrevealedbyanakselectivechannel[J]. proteincell,2018,009(007):629. |
| APA | Yu Jie.,Zhang Bing.,Zhang Yixiao.,Xu Congqiao.,Zhuo Wei.,...&Yang Maojun.(2018).abindingblockionselectivemechanismrevealedbyanakselectivechannel.proteincell,009(007),629. |
| MLA | Yu Jie,et al."abindingblockionselectivemechanismrevealedbyanakselectivechannel".proteincell 009.007(2018):629. |
入库方式： OAI收割
来源：上海药物研究所
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