liquidchromatographytandemmassspectrometrysimultaneousdeterminationofrepaglinideandmetformininhumanplasmaanditsapplicationtobioequivalencestudy
文献类型：期刊论文
| 作者 | Liang Xiaorong1; Dai Xiaojian2 ; Zhang Yifan2; Ding Juefang2; Chen Xiaoyan2; Zhong Dafang1
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| 刊名 | 药学学报
 |
| 出版日期 | 2013 |
| 卷号 | 48期号:4页码:547 |
| 关键词 | 瑞格列奈 二甲双胍 |
| ISSN号 | 0513-4870 |
| 英文摘要 | A simple, sensitive, selective, and reproducible liquid chromatography-tandem mass spectrometric method was developed for the simultaneous determination of repaglinide and metformin in human plasma using d_5-repaglinide and d_6-metformin as internal standards (ISs). After a simple protein precipitation using acetonitrile as the precipitation solvent, both analytes and ISs were separated on a Venusil ASB C_(18) (150 mm × 4.6 mm, 5 μm) via gradient elution using acetonitrile ? 10 mmol·L~(?1) ammonium acetate as the mobile phase. A chromatographic total run time of 7.5 min was achieved. Mass spectrometric detection was conducted with atmospheric pressure chemical ionization under positive-ion and multiple-reaction monitoring modes. The method was linear over the 0.2 to 60.0 ng·mL~(?1) concentration range for repaglinide and over the 4 to 1 000 ng·mL~(?1) range for metformin. For both analytes, the intra- and inter-accuracies and precisions were within the ±15% acceptable limit across all concentrations. The validated method was successfully applied to a clinical bioequivalence study. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/287802]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.浙江工业大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Liang Xiaorong,Dai Xiaojian,Zhang Yifan,et al. liquidchromatographytandemmassspectrometrysimultaneousdeterminationofrepaglinideandmetformininhumanplasmaanditsapplicationtobioequivalencestudy[J]. 药学学报,2013,48(4):547. |
| APA | Liang Xiaorong,Dai Xiaojian,Zhang Yifan,Ding Juefang,Chen Xiaoyan,&Zhong Dafang.(2013).liquidchromatographytandemmassspectrometrysimultaneousdeterminationofrepaglinideandmetformininhumanplasmaanditsapplicationtobioequivalencestudy.药学学报,48(4),547. |
| MLA | Liang Xiaorong,et al."liquidchromatographytandemmassspectrometrysimultaneousdeterminationofrepaglinideandmetformininhumanplasmaanditsapplicationtobioequivalencestudy".药学学报 48.4(2013):547. |
入库方式： OAI收割
来源：上海药物研究所
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