effectsofscutellarinonpkcinpc12cellinjuryinducedbyoxygenandglucosedeprivation
文献类型：期刊论文
| 作者 | Wei XU; Ruopeng ZHA; Wenyi WANG; Yiping WANG
|
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2007 |
| 卷号 | 028期号:010页码:1573 |
| 关键词 | 黄芩素 细胞凋亡 钙 蛋白质 |
| ISSN号 | 1671-4083 |
| 英文摘要 | Aim： To evaluate the neuroprotective effect and mechanisms of scutellarin （Scu） against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion （OGD-Rep）. Methods： Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion （OGD- Rep）, used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide （MTT） assay and the amount of LDH release was determined using assay kits. Ca^2＋i was monitored using a fluorescent Ca^2＋ - sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C （PKC）T was determined usiiag both RT-PCR and Western blotting. The translocation of PKCT was assayed by subcellular fractionation and Western blotting. Results： OGD-Rep injury significantly elevated the level of LDH release, Ca^2＋i, mRNA expression and the translocation of PKCγ compared in the PC12 cells with those of the normal group. Scu （10-100 μmol/L） exerted a protective effect against OGD-Rep injury by reducing LDH release, Ca^2＋i, the percent of apoptosis, and the translocation of PKCγ. Conclusion： Scu inhibits the increase of Ca^2＋i and the activation of PKCγ, exerting protective effects against PC12 cell injury induced by OGD-Rep. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/287845]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Wei XU,Ruopeng ZHA,Wenyi WANG,et al. effectsofscutellarinonpkcinpc12cellinjuryinducedbyoxygenandglucosedeprivation[J]. actapharmacologicasinica,2007,028(010):1573. |
| APA | Wei XU,Ruopeng ZHA,Wenyi WANG,&Yiping WANG.(2007).effectsofscutellarinonpkcinpc12cellinjuryinducedbyoxygenandglucosedeprivation.actapharmacologicasinica,028(010),1573. |
| MLA | Wei XU,et al."effectsofscutellarinonpkcinpc12cellinjuryinducedbyoxygenandglucosedeprivation".actapharmacologicasinica 028.010(2007):1573. |
入库方式： OAI收割
来源：上海药物研究所
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