inductionoflivercytochromep4501a2expressionbyflutamideinrats
文献类型：期刊论文
| 作者 | Wang Haixue1; Liu Xiao1; Xu Changjiang1; Ma Xiaochao2; Long Jianer1; Li Duan1 |
| 刊名 | actapharmacologicasinica
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:11页码:1382 |
| 关键词 | NONSTEROIDAL ANTIANDROGEN SCH 13521 METABOLISM THERAPY HEPATOTOXICITY CAFFEINE FAILURE ENZYME flutamide cytochrome P450 1A2 induction |
| ISSN号 | 1671-4083 |
| DOI | 10.1111/j.1745-7254.2005.00211.x |
| 英文摘要 | Aim: To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Methods: Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP 1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate. Results: CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity. Conclusion: Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288001]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.复旦大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Wang Haixue,Liu Xiao,Xu Changjiang,et al. inductionoflivercytochromep4501a2expressionbyflutamideinrats[J]. actapharmacologicasinica,2005,26(11):1382. |
| APA | Wang Haixue,Liu Xiao,Xu Changjiang,Ma Xiaochao,Long Jianer,&Li Duan.(2005).inductionoflivercytochromep4501a2expressionbyflutamideinrats.actapharmacologicasinica,26(11),1382. |
| MLA | Wang Haixue,et al."inductionoflivercytochromep4501a2expressionbyflutamideinrats".actapharmacologicasinica 26.11(2005):1382. |
入库方式： OAI收割
来源：上海药物研究所
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