amideexchangerateeditednmraerenmrexperimentanovelmethodforresolvingoverlappingresonances
文献类型：期刊论文
| 作者 | XueHu Liu1; DongHai Lin2 |
| 刊名 | chinesejournalofchemistry
 |
| 出版日期 | 2007 |
| 卷号 | 25期号:3页码:411 |
| 关键词 | SOLVENT-EXPOSED AMIDES PROTEINS GRADIENT TROSY WATER NMR resonance overlapping amide-exchange-rate-edited SEA presat TROSY |
| ISSN号 | 1001-604X |
| 英文摘要 | This paper describes an amide-exchange-rate-edited (AERE) NMR method that can effectively alleviate the problem of resonance overlap for proteins and peptides. This method exploits the diversity of amide proton exchange rates and consists of two complementary experiments: (1) SEA (solvent exposed amide)-type NMR experiments to map exchangeable surface residues whose amides are not involved in hydrogen bonding, and (2) pre-sat-type NMR experiments to map solvent inaccessibly buried residues or nonexchangeable residues located in hydrogen-bonded secondary structures with properly controlled saturation transfer via amide proton exchanges with the solvent. This method separates overlapping resonances in a spectrum into two complementary spectra. The AERE-NMR method was demonstrated with a sample of N-15/C-13/H-2(70%) labeled ribosome-inactivating protein trichosanthin of 247 residues. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288154]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.北京大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | XueHu Liu,DongHai Lin. amideexchangerateeditednmraerenmrexperimentanovelmethodforresolvingoverlappingresonances[J]. chinesejournalofchemistry,2007,25(3):411. |
| APA | XueHu Liu,&DongHai Lin.(2007).amideexchangerateeditednmraerenmrexperimentanovelmethodforresolvingoverlappingresonances.chinesejournalofchemistry,25(3),411. |
| MLA | XueHu Liu,et al."amideexchangerateeditednmraerenmrexperimentanovelmethodforresolvingoverlappingresonances".chinesejournalofchemistry 25.3(2007):411. |
入库方式： OAI收割
来源：上海药物研究所
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