identificationofaoscbindingproteinsinneurons
文献类型：期刊论文
| 作者 | LIU Ming1; NIE Qin1; XIN Xianliang2; GENG Meiyu1 |
| 刊名 | chinesejournalofoceanologyandlimnology
 |
| 出版日期 | 2008 |
| 卷号 | 26期号:4页码:394 |
| 关键词 | OLIGOSACCHARIDE SUGAR CHAIN AMYLOID PRECURSOR PROTEIN ACIDIC OLIGOSACCHARIDE BETA-PROTEIN CLATHRIN APP AOSC Alzheimer's disease marine oligosaccharide binding protein(s) |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-008-0394-8 |
| 英文摘要 | Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brown algae polysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer's Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities. |
| 资助项目 | [National Basic Research Program of China (973 Program)] ; [Natural Science Foundation of China for Distinguished Young Scholars] ; [Natural Science Foundation of China] ; [Chinese High technology Research and Development Program of China(863 Program)] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288387]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.中国海洋大学 2.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | LIU Ming,NIE Qin,XIN Xianliang,et al. identificationofaoscbindingproteinsinneurons[J]. chinesejournalofoceanologyandlimnology,2008,26(4):394. |
| APA | LIU Ming,NIE Qin,XIN Xianliang,&GENG Meiyu.(2008).identificationofaoscbindingproteinsinneurons.chinesejournalofoceanologyandlimnology,26(4),394. |
| MLA | LIU Ming,et al."identificationofaoscbindingproteinsinneurons".chinesejournalofoceanologyandlimnology 26.4(2008):394. |
入库方式： OAI收割
来源：上海药物研究所
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